Genomic characteristics of hepatitis A virus epidemic strains in Zhuanghe from 2020 to 2021
10.13200/j.cnki.cjb.004215
- VernacularTitle:2020—2021年庄河市甲型肝炎病毒流行株基因组特征分析
- Author:
SHAO Yuping
- Publication Type:Journal Article
- Keywords:
Hepatitis A virus(HAV);
Molecular epidemiology;
Environmental sewage
- From:
Chinese Journal of Biologicals
2024;37(5):554-558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genomic characteristics of hepatitis A virus(HAV)in environmental sewage in Zhuanghe from 2020 to 2021 and compare them with those in case samples.Methods Eighteen sewage samples(environmental sewage)were collected from the entry point of the sewage treatment plant in Zhuanghe from July to December 2021,and the viral RNA was extracted. After reverse transcription,VP1-2A region was amplified by nested PCR,sequenced and spliced.The five HAV nucleotide sequences(321 bp)obtained were analyzed for phylogenetic tree by using bioinformatics software.Results The HAV of environmental sewage samples in 2021 were all 1A subtype,and the nucleotide sequence homology of VP1-2A region was 98. 7%-100%. The nucleotide sequence homology was 97. 8%-100% with that of the hepatitis A case samples from Zhuanghe in 2020,which could be evolutionarily divided into two branches.Conclusion Subtype 1A was the dominant genotype of HAV in environmental sewage in Zhuanghe,which was different from the genome of the case sample,and also had the same genome of some strains. By comparing the similarities between HAV nucleic acid in environmental sewage and that in case samples,we can better understand the circulation of HAV,so as to make an early warning for the epidemiological monitoring of hepatitis A.
