Study on in vitro anti-inflammatory effects and mechanisms of oblongifolins A extracted from Garcinia oblon-gifolia
- VernacularTitle:岭南山竹子提取物oblongifolinsA的体外抗炎作用及机制研究
- Author:
Xueshan LI
1
;
Guiming QIN
2
;
Huiying SHI
1
;
Xiaoqin ZOU
3
;
Jie FENG
4
;
Xiaobin ZHONG
1
Author Information
1. Dept. of Pharmacy，the Second Affiliated Hospital of Guangxi Medical University，Nanning 530007，China
2. Drug Clinical Trial Facility Office，the Second Nanning People’s Hospital，Nanning 530031，China
3. Dept. of Scientific Research，the First Affiliated Hospital of Guangxi Medical University，Nanning 530021，China
4. TCM Teaching and Research Section，School of Pharmaceutical Sciences，Guangxi Medical University，Nanning 530021，China
- Publication Type:Journal Article
- Keywords:
oblongifolins A;
inflammation;
NF-κB signaling pathway;
Nrf2 signaling pathway
- From:
China Pharmacy
2024;35(10):1209-1214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the in vitro anti-inflammatory effects and mechanisms of oblongifolins A （OA） extracted from Garcinia oblongifolia. METHODS RAW264.7 cells were used as the research subject and divided into control group （0.5% DMSO）， lipopolysaccharide （LPS） group （1 μg/mL）， DEX group （10 µmol/L DEX+1 μg/mL LPS）， and low-， medium-， and high-concentration groups of OA （7.5， 15， 30 µmol/L OA+1 μg/mL LPS）. Except for the control group， the remaining groups were first stimulated with LPS for 1 hour and then mixed with drugs for 24 hours. The morphological changes of cells were observed in each group. The contents of nitric oxide （NO）， reactive oxygen species （ROS）， tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6）， IL-1β， IL-4 and IL-10 were detected in cells of each group； mRNA expression levels of TNF-α， IL-6 and IL-1β were measured. The expression of key proteins in the nuclear factor κB （NF-κB） and nuclear factor-erythroid 2-related factor 2 （Nrf2） signaling pathways in each group， as well as the nuclear translocation of NF-κB p65 and Nrf2 proteins in control group， LPS group and OA high-concentration group， were detected. RESULTS Compared to the LPS group， the number of spindle-shaped and irregular cells gradually decreased in OA groups， the contents of NO， ROS （except for OA low-concentration group）， TNF-α， IL-6 and IL-1β， the mRNA expressions of TNF-α， IL-6 （except for OA low-concentration group） and IL-1β as well as the protein expressions of phosphorylated NF-κB p65 （p-NF-κB p65）， p-IκBα， and Kelch-like ECH-associated protein 1 （Keap1） were decreased significantly （P＜0.05）. The contents of IL-4 and IL-10， protein expressions of IκBα， Nrf2 （except for OA low- and medium-concentration groups）， HO-1 （except for OA low-concentration group） and NQO1 were all increased significantly （P＜0.05）. OA of high concentration could inhibit NF-κB p65 protein nuclear translocation and promote Nrf2 protein nuclear translocation. CONCLUSIONS OA can suppress LPS-induced inflammation in RAW264.7 macrophages. The underlying molecular mechanism likely entails the inhibition of the NF-κB signaling pathway， the activation of the Nrf2 signaling pathway and the reduction of ROS and inflammatory factor release.
