Effects and mechanism of Yiqi huoxue decoction on lumbar disc herniation in rats
- VernacularTitle:益气活血方对大鼠腰椎间盘突出的影响及机制
- Author:
Xue BAI
1
,
2
;
Xiaoxian SUN
2
;
Yang GUO
2
;
Yong MA
1
,
2
;
Mengmin LIU
1
;
Hong JIANG
3
;
Shun LIN
3
;
Rui CAO
4
;
Yongfeng YUAN
5
;
Jintao LIU
3
Author Information
1. School of Integrated Chinese and Western Medicine，Nanjing University of Chinese Medicine，Nanjing 210023，China
2. Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology，Nanjing University of Chinese Medicine，Nanjing 210023，China
3. Dept. of Orthopedics and Traumatology，Suzhou Hospital of Traditional Chinese Medicine，Nanjing University of Chinese Medicine，Jiangsu Suzhou 215008，China
4. Dept. of Spinal Orthopedics，Taizhou Jiangyan Hospital of Traditional Chinese Medicine，Jiangsu Taizhou 225300，China
5. Dept. of Radiology，the Affiliated Hospital of Nanjing University of Chinese Medicine，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
lumbar disc herniation;
inflammatory response;
NF-κB signaling pathway;
autophagy
- From:
China Pharmacy
2024;35(10):1186-1192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects and mechanism of Yiqi huoxue decoction （YQHX） on lumbar disc herniation in rats. METHODS Rats were randomly divided into sham operation group， model group， NF-κB inhibitor group （QNZ group， 1 mg/kg）， YQHX group （9.1 g/kg） and combination group （YQHX+QNZ group， the same dose as each single drug group）， with 10 rats in each group. Except for sham operation group， lumbar disc herniation model of rats was induced in other groups； administration groups were given QNZ intraperitoneally or/and YQHX intragastrically， once a day， for 8 consecutive weeks. The severity of intervertebral disc herniation was evaluated in each group； the pathological changes of intervertebral discs and the changes of autophagy of nucleus pulposus cells were all observed； the level of tumor necrosis factor-α （TNF-α） in serum， and the ratios of Bcl-2/adenovirus E1B interacting protein 3 （BNIP3） and Beclin-1 positive cells in intervertebral disctissues were detected； the phosphorylation of nuclear factor-κB （NF-κB） p65， the expressions of tumor necrosis factor receptor- associated factor-2 （TRAF-2）， TRAF-3， BNIP3 and LC3B protein， and mRNA expressions of NF-κB p65， LC3B， p62，BNIP3 and Beclin-1 were determined. RESULTS Compared with model group， Pfirrmann grading score decreased significantly，the pathological injury of intervertebral disc tissue was relieved in YQHX group； the number of autophagosomes in nucleus pulposus cells increased； serum level of TNF-α and mRNA expression of p62 in intervertebral disc tissue decreased significantly； the ratios of BNIP3 and Beclin-1 positive cells， the phosphorylation of NF-κB p65， the expressions of TRAF-2， TRAF-3， BNIP3 and LC3B protein as well as the mRNA expressions of NF- κB p65， LC3B， BNIP3 and Beclin-1 decreased significantly in intervertebral disc tissues （P＜0.05）. The changes of above indexes in YQHX group were reversed partly in YQHX+QNZ group. CONCLUSIONS YQHX promotes the elevation of autophagy level of intervertebral discs， slows down the inflammatory response and the progression of lumbar disc herniation by activating the NF-κB signaling pathway.
