Establishment of Ischemia-Reperfusion Model in Cynomolgus Macaques and Effects of Edaravone Intervention
10.12300/j.issn.1674-5817.2022.165
- VernacularTitle:食蟹猴缺血再灌注模型的建立及依达拉奉干预作用
- Author:
Mengxian PAN
1
;
Xiaojiao HUANG
1
;
Zhongli HUANG
1
;
Guo SHEN
1
;
Pengfei ZHANG
1
;
Yong ZENG
1
;
Wenfeng LI
2
;
Huabo ZHOU
3
;
Zhumei WEI
1
Author Information
1. Guangxi Gui Academy of Science Huaren Jiying Biotechnology Co., LTD., Nanning 530000, China
2. Guangxi Thinxon Biomedical Technology Co., LTD., Nanning 530000, China
3. Nanning Huabo Pet Hospital, Nanning 530000, China
- Publication Type:Journal Article
- Keywords:
Cynomolgus macaques;
Ischemic stroke;
Ischemia-reperfusion;
Edaravone
- From:
Laboratory Animal and Comparative Medicine
2023;43(2):136-144
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish an ischemia-reperfusion model in cynomolgus macaques and to analyse the effects of edaravone intervention. MethodsA total of fifteen adult male cynomolgus macaques were randomly divided into three groups: sham operation (Sham group, n=3), ischemia-reperfusion model (Model group, n=6) and edaravone treatment (Edaravone group, n=6). Ischemic-reperfusion model of cynomolgus macaques was established by clamping the M1 branch of the left cerebral artery for 1 h. After 2 h of reperfusion, the animals in Edaravone group were injected with 0.5 mg/kg edaravone intravenously for intervention treatment, while the animals in Sham and Model groups were injected with an equal volume of normal saline intravenously, twice a day, from the 2nd to 7th day. The behavioral video recordings, clinical observations and neurological deficit scores of cynomolgus macaques were obtained, and brain edema volume and cerebral ischemia volume were statistically analyzed. ResultsCompared with the Sham group, the animals in Model group showed typical symptoms of ischemic stroke, with a significant increase in the neurological deficit score， the volumes of edema and infarct of brain tissue (all P＜0.01). Compared with Model group, the neurological deficit score, the volumes of edema and infarct of brain tissue were significantly reduced in Edaravone group (all P＜0.05). ConclusionAn animal model of ischemia-reperfusion in cynomolgus macaques was successfully established, and edaravone was confirmed to alleviate the damage caused by ischemia-reperfusion.
