Expression of IL-10, TGF-beta1 and TNF-alpha in Cultured Keratinocytes (HaCaT Cells) after IPL Treatment or ALA-IPL Photodynamic Treatment.
- Author:
Ji Yeon BYUN
1
;
Hae Young CHOI
;
Ki Bum MYUNG
;
You Won CHOI
Author Information
- Publication Type:Original Article
- Keywords: IL-10; Keratinocyte; Photodynamic therapy; TGF-beta1; TNF-alpha
- MeSH: Cell Death; Enzyme-Linked Immunosorbent Assay; Interleukin-10; Keratinocytes; Light; Photochemotherapy; Psoriasis; RNA, Messenger; Skin Diseases; Transforming Growth Factor beta1; Triazenes; Tumor Necrosis Factor-alpha
- From:Annals of Dermatology 2009;21(1):12-17
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Depending on the light dose and concentration of photosensitizer for photodynamic treatment (PDT), a multitude of dose-related events are demonstrable in PDT-treated cells. Sublethal doses may result in the alteration of cytokine release and consequently modify immune actions, rather than cause cell death. OBJECTIVE: The purpose of this study was to investigate cytokine expression in cultured HaCaT cells after intense pulse light (IPL) treatment or PDT utilizing 5-aminolevulinic acid (ALA) and IPL at sublethal doses. METHODS: Cultured HaCaT cells were treated with either IPL only (4, 8 and 12 J/cm2) or ALA-IPL PDT (100micromol/L of ALA; 0, 4, 8, and 12 J/cm2 of IPL). The expression of IL-10, TGF-beta1 and TNF-alpha was investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. RESULTS: IL-10 protein increased up to 5.95-fold after IPL treatment and up to 2.85-fold after PDT. TGF-beta1 mRNA and protein showed slight increases after both IPL treatment and PDT, of which the latter induced slightly larger increases. TNF-alpha mRNA and protein showed no induction or reduction after PDT. CONCLUSION: Increased expressions of IL-10 and TGF-beta1 was observed after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses, and that of TGF-beta1 may be related to the therapeutic effect for psoriasis. The finding that IL-10 induction was more marked after IPL treatment than after PDT suggests that other mechanisms than IL-10 induction in keratinocytes after PDT may participate in the anti-inflammatory effect of PDT.
