Effects of Pogostemon cablin on Serum Metabolomice of Guizhou Miniature Pigs and It's mechanism
10.12300/j.issn.1674-5817.2022.186
- VernacularTitle:广藿香对贵州小型猪血清代谢组的影响及其机制探讨
- Author:
Taofeng LU
1
;
Hui ZHANG
2
;
Jie ZHOU
3
;
Qian LI
1
;
Shuguang WU
1
;
Yanjun WU
1
Author Information
1. Institute for Laboratory Animal Research, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
2. Dongguan Hospital, Guangzhou University of Traditional Chinese Medicine, Dongguan 523000, China
3. Shanghai Yishang Biotechnology Co., LTD., Shanghai 201319, China
- Publication Type:Journal Article
- Keywords:
Serum metabolomics;
Pogostemon cablin;
Guizhou miniature pig;
Pharmacology of traditional Chinese medicine;
Action mechanism
- From:
Laboratory Animal and Comparative Medicine
2023;43(3):253-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective Based on the liquid chromatography-tandem mass spectormetry (LC-MS/MS), to study the effects of Pogostemon cablin on serum metabolism of Guizhou miniature pigs， and to explore its pharmacological mechanism.MethodsNine healthy Guizhou miniature pigs were divided into two groups, namely Pogostemon cablin drug group (n=5) and control group (n=4). The pigs in Pogostemon cablin drug group were orally fed with traditional Chinese medicine formula granules, each 0.5 g per day, for consecutive 8 days, while those in control group were given normal feeding without additional treatment. After the feeding experiment, serum samples were collected and analyzed using the LC-MS/MS technology. The metabolomics data was annotated and compared with KEGG, HMDB and Lipidmaps databases. Bioinformatics analysis methods including partial least squares discriminant analysis (PLS-DA), intergroup clustering, differential metabolite analysis and functional enrichment were used to screen differential metabolic biomarkers and their possible metabolic pathways.Results Forty-four differential metabolites (P<0.05) were screened out from the 443 metabolites, eight differential metabolites were significantly up-regulated (P<0.01), namely cinnamoylglycine, N-benzyl-N-isopropyl-N'-[4-(trifluoromethoxy) phenyl]urea, hypotaurine, D-glucose 6-phosphate, cis-2-decenoic acid, 11(Z),14(Z)-eicosadienoic acid, prostaglandin A2 and 10-hydroxydecanoic acid, and three differential metabolites were significantly down-regulated (P<0.01), namely lysophosphatidyl choline 22:5, lysophosphatidic acid 22:6 and lysophosphatidic acid 22:5. The differential metabolites were mainly enriched in the metabolic pathways of alanine, aspartate and glutamate metabolism (MapID: map00250) and taurine and hypotaurine metabolism (MapID: map00430).Conclusion Pogostemon cablin can significantly affect the metabolism of lysophosphatidic acids in porcine, and relieve the disorder of amino acids metabolism and regulate the occurrence of inflammation by affecting the metabolic pathways of alanine, aspartate and glutamate metabolism and taurine and hypotaurine metabolism.
