Evaluation and Analysis of Modeling Methods for Animal Models with Diminished Ovarian Reserve
10.12300/j.issn.1674-5817.2023.032
- VernacularTitle:卵巢储备功能减退动物模型的造模方法评价与分析
- Author:
Hui HUANG
1
;
Yasheng DENG
1
;
Tianwei LIANG
1
;
Yiqing ZHENG
1
;
Yanping FAN
1
;
Na RONG
1
;
Jiang LIN
1
Author Information
1. School of Basic Medical Sciences, Guangxi University of Traditional Chinese Medicine, Nanning 530200, China
- Publication Type:Journal Article
- Keywords:
Diminished ovarian reserve;
Animal model;
Literature analysis;
Model evaluation;
Rat;
Mouse
- From:
Laboratory Animal and Comparative Medicine
2023;43(4):422-428
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analysis the modeling characteristics of diminished ovarian reserve (DOR) animal models, and provide the reference for the standardization of DOR animal models.MethodsThe research articles on DOR animal models were retrieved. Microsoft Excel 2010 software was used to summarize the experimental animal species, modeling methods, modeling cycles, high-frequency detection indexes and types of positive drugs documented in the literature, and the data results were evaluated and analyzed by NoteExpress software.ResultsA total of 93 research articles on DOR that met the criteria were enrolled. And it was found that, SD rat was the most frequently used animal type (68 times, 73.12%), followed by C57BL/6 mouse (13 times, 13.98%), while the tripterygium wilfordii treatment was the most frequently used modeling method (38 times, 40.86%), followed by the cyclophosphamide treatment (28 times, 30.11%) for DOR animal models. The high frequency detection indicators were vaginal exfoliation cytology detection (93 times, 23.97%) and HE staining to observe histopathological changes (72 times, 18.56%). Among these 64 research articles containing positive control drugs, the most frequently used western drug was estrogen (50 times,62.50%) and Chinese Traditional medicine was Kuntai capsules (2 times, 2.50%).ConclusionsSD rats are mostly used to induce modeling in animal experiments on DOR through tripterygium wilfordii and cyclophosphamide, which can effectively improve the experimental efficiency. It is recommended to integrate the results of ovarian histopathology and serum biochemical indexes for model assessment.
