Application of Optimized Latex Perfusion Technique in the Establishment of Craniofacial Venous Model in Mice
10.12300/j.issn.1674-5817.2023.095
- VernacularTitle:乳胶血管灌注技术制作小鼠头面部静脉血管模型方法初探
- Author:
Chengji WANG
1
;
Jue WANG
1
;
Haijie WANG
1
;
Weisheng LU
1
;
Yan SHI
2
;
Zhengye GU
1
;
Mingqiu WAN
1
;
Ruling SHEN
1
Author Information
1. Shanghai Laboratory Animal Research Center, Shanghai 201203,China
2. Mayo Pet (Shanghai) Co., Ltd.,Shanghai 200235, China
- Publication Type:Journal Article
- Keywords:
Latex perfusion;
Vascular model;
Craniofacial vessels in mice
- From:
Laboratory Animal and Comparative Medicine
2023;43(5):574-578
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveOptimize the latex perfusion technique and apply it to the construction of a murine craniofacial venous vascular model.Methods A total of nine 8-week-old male C57BL/6 mice weighing (25.0±1.3) g were randomly divided into three groups: 60% latex physiological saline group, 60% latex heparin group, and 30% latex heparin group. After completion of the perfusion, the specimens were immersed in 4 °C formalin fixative for 24 h, followed by dissection, observation, and measurement of the extracranial blood vessel diameters. Results After 200 μL latex perfusion solution was injected into the external jugular vein, the supraorbital vein, infraorbital vein, temporal vein, retrofacial vein, masseter vein and external jugular vein were perfused in each group.After comparing the perfusion degree of the distal branches of blood vessels, sublingual vein and tip venule, it was found that the 30% latex heparin group had the best perfusion effect, followed by the 60% latex heparin group, and the 60% latex saline group had the worst perfusion effect.ConclusionThe optimized latex perfusion technique can effectively infuse the veins in the head and face of mice, and this technique can provide a good reference for the study of the direction and morphology of facial veins in mice.
