Acetaminophen content assay with UHPLC in the sustained-release tablet exposed to radiation
10.12206/j.issn.2097-2024.202109100
- VernacularTitle:UHPLC法测定辐射后对乙酰氨基酚缓释片含量
- Author:
Dexun YU
1
;
Xinhui HUANG
1
;
Kehan ZHU
1
;
Ting HUANG
1
;
Tingting ZHOU
1
;
Jianyi GAO
2
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. China Astronaut Research and Training Center, Beijing 100094, China.
- Keywords:
acetaminophen;
UHPLC;
content;
radiation
- From:
Journal of Pharmaceutical Practice and Service
2022;40(6):550-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assay the contents of acetaminophen with ultra high-performance liquid chromatography (UHPLC) method in the sustained-release tablets radiated by Gamma ray. Methods Acetaminophen sustained-release tablets were radiated by 60Co. UHPLC equipped with the Shim-pack GISS-C18（2.1 mm×50 mm, 1.9 μm）was used for the assay. The mobile phase was methanol-0.05 % ammonium acetate solution (15∶85). The flow rate was 0.3 ml/min with the detection wavelength at 245 nm. Results The acetaminophen showed good linear relationship within the range of 20-100 μg/ml (r=0.999 4). The RSD values of repeatability was 0.9 %. The average recovery was 97.9 %-104.9 %. Acetaminophen content was 96.2 %, 92.2 %, 91.8 %, 83.9 % at 0, 8, 50 and 80 kGy radiation, respectively. Conclusion This method is speedy and accurate. It can be used to assay the content of acetaminophen in the sustained-release tablets after radiation. The content of acetaminophen decreased after radiation.
