Study on quality standard improvement for Gansu granules
10.12206/j.issn.2097-2024.202207061
- VernacularTitle:肝苏颗粒质量标准提升研究
- Author:
Chaoyang TAO
1
;
Pengjie SHI
2
;
Xin ZHOU
2
;
Lianna SUN
2
Author Information
1. Pharmaceutical Preparation Research Center of General Hospital of Tibet Military Region, Lhasa 850007, China.
2. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
- Keywords:
Gansu granules;
quality standards;
TLC;
pinocembrin-7-O-β-D-glucoside;
HPLC
- From:
Journal of Pharmaceutical Practice and Service
2022;40(6):546-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard of Gansu granules (GGs) and effectively control the quality of GGs. Methods TLC identification method of Gansu granules was improved by using pinocembrin-7-O-β-D-glucoside as the indicator. In HPLC analysis, Agilent ZorbaxSB-C18 (4.6 mm×250 mm, 5 μ m) was used as a chromatographic column, and acetonitrile-0.5% formic acid solution was used as the mobile phase. The detection wavelength of 280 nm was used to analyze the content of pinocembrin-7-O-β-D-glucoside in GGs. Results The separation of characteristic spots was good and clear when the established TLC method was used to identify Gansu granules. Quantitative analysis showed that the concentration of pinocembrin-7-O-β-D-glucoside has a good linear relationship with the peak area in the range of 2.4-240 µg/ml (r= 0.9999). The average sample recovery rate of pinocembrin-7-O-β-D-glucoside is 100.76% with RSD value of 0.66%. The accuracy was good. Conclusion The TLC identification method and HPLC content determination method established in this experiment have strong specificity and good reproducibility, and can be used as an improved standard for the quality control of Gansu granules.
