The acute toxicity of tetrodotoxin to zebra fish
10.12206/j.issn.2097-2024.202202083
- VernacularTitle:河豚毒素对斑马鱼的急性毒性研究
- Author:
Haoneng WANG
1
;
Qianzhu ZHANGJI
2
;
Jingfeng LI
2
;
Rui JIA
3
;
Yuping ZHU
4
;
Jiangbo ZHU
2
;
Jikuai CHEN
2
;
Lang YAN
2
Author Information
1. Department of Marine Radiation Medicine, Naval Medical University, Shanghai 20043, China.
2. Department of Health Toxicology, Naval Medical University, Shanghai 200433, China.
3. College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China.
4. Basic Medical Experimental Teaching Center, Basic Medical College, Naval Medical University, Shanghai 200433, China.
- Keywords:
tetrodotoxin;
zebra fish;
acute toxicity
- From:
Journal of Pharmaceutical Practice and Service
2022;40(6):536-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the acute toxicity of tetrodotoxin to zebra fish. Methods The maximum non-lethal concentration (MNLC) and 10% lethal concentration (LC10) determinations were used to assess the acute toxicity of tetrodotoxin. Results According to the simulation calculation of Origin 8.0 software, the MNLC was 8.62 µmol/L and 15.2 µmol/L for LC10. Under the experimental conditions, tetrodotoxin at a concentration of 16.0 µmol/L and above induced pericardial edema and arrhythmia, leading to the death of zebra fish. The target organs for acute toxicity of tetrodotoxin were the heart and liver. The main manifestations were pericardial edema, arrhythmia, and delayed yolk sac absorption. The toxicity appeared at a concentration of 0.958 µmol/L. Conclusion Tetrodotoxin has heart and liver toxicity to zebra fish, and its toxicity is dose-dependent.
