Study on polymorphism of peritoneal microbial community after laparoscopic exploration in New Zealand rabbits based on 16S rDNA sequencing
10.12206/j.issn.2097-2024.202201016
- VernacularTitle:基于16S rDNA测序研究新西兰兔腹腔镜探查术后腹腔微生物群落多态性改变
- Author:
Pingping ZHANG
1
;
Wenbin WU
1
;
Qi CAO
1
;
Zhuo QU
2
;
Pei WANG
1
Author Information
1. Department of Pharmacology, School of Pharmacy, Naval Medical University , Shanghai 200433, China.
2. College of Pharmacy, Ningxia Medical University, Yinchun 750000, China.
- Keywords:
16S rDNA sequencing;
laparoscope;
microbial community polymorphism
- From:
Journal of Pharmaceutical Practice and Service
2022;40(6):494-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the polymorphism of microbial community after laparoscopic abdominal exploration by using bacterial 16s ribosomal DNA (16S rDNA) sequencing technology. Methods New Zealand rabbits were divided into model group and control group. The rabbits in the model group were operated by portable laparoscopy, and the rabbits in the control group were not treated. One week later, the peritoneal effusions of the model group and the control group were taken for 16S rDNA sequencing to analyze the microbial community polymorphism. To explore the changes of microbial community in peritoneal effusion in the model group compared with the control group. Results After 16S rDNA sequencing, bioinformatics was used to determine the microbial communities. Inter group difference analysis showed a good similarity of microbial communities between the two groups. OTU taxonomic analysis and species composition analysis (Rank-Abundance curve and Venn diagram) found that the microbial community level of the model group was significantly higher than that of the control group. Alpha diversity analysis (Sobs, Ace, Shannon, Simpson) showed that the richness and diversity of microbial community in the model group were higher than those in the control group. Microbial composition analysis showed that the number of miscellaneous bacteria in the model group increased by about 30% compared with the control group. The species differences between the two groups were tested for significance. It was found that Pasteurellales, Neisseria and Tsukamurella increased significantly. Conclusion The diversity of microbial communities in peritoneal effusion increases after laparoscopic abdominal exploration in New Zealand rabbits, and the most significant increases are Pasteurella, Neisseria and Tsukamura.
