Analysis of tissue distribution of metabolites in Crocus sativus L. corms based on DESI mass spectrometry imaging technique
10.12206/j.issn.2097-2024.202310055
- VernacularTitle:基于解吸电喷雾质谱成像技术分析番红花球茎中代谢物的组织分布
- Author:
Xi CHEN
1
;
Xingguang XIE
1
;
Kunmiao FENG
1
;
Yingjie LU
1
;
Ting HAN
1
Author Information
1. Department of Pharmacognosy, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
Crocus sativus L. corms;
desorption electrospray ionization mass spectrometry imaging;
different origins;
different parts of C. sativus corms;
metabolite distribution
- From:
Journal of Pharmaceutical Practice and Service
2024;42(4):169-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the distribution characteristics of endogenous metabolites in Crocus sativus L. corms from different origins. Methods A method based on desorption electrospray ionization mass spectrometry imaging and optimized sample pretreatment was developed for directly visualize metabolites in C. sativus corms. Results In situ characterization of metabolites such as flavonoids, organic acids, amino acids, carotenoids, and cyclic enol ether terpene glycosides was achieved. L-Citruline, phenylacetylglycine, sativol, and geniposide were specifically distributed in the corms. Apigenin 7-（6''-O-acetyl）-glucoside, isorhamnetin-3-O-β-D-Glucoside, dhurrin 6'-glucoside, and Apigenin 7-O-diglucuronide were mainly distributed in the terminal bud. For compounds distributed in the corms, the highest abundance was found in corms from Shanghai, followed by Zhejiang and the lowest from Anhui. Conclusion The distribution of metabolites in different parts of C. sativus corms from different origins and the same origin varies significantly. Flavonoids and flavonoid derivatives such as isorhamnetin-3-O-β-D-Glucoside and apigenin derivatives are mainly distributed in the terminal buds, in addition, the natural plant protection agent dhurrin 6'-glucoside is also mainly distributed in the terminal corms, whereas amino acids, which are used as energy and material supplies, are mainly accumulated in the corms.
