Study on antifungal effect of demethylzelamaldehyde in vitro
10.12206/j.issn.2097-2024.202310011
- VernacularTitle:去甲泽拉木醛的体外抗真菌作用研究
- Author:
Lei HAN
1
;
Hua ZHONG
2
,
3
;
Xinrong WANG
4
;
Yan WANG
2
,
3
,
5
Author Information
1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China
3. Innovation Center for Medical Basic Research on Fungal Infectious Diseases, Ministry of Education, Shanghai 200433, China.
4. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
5. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
- Keywords:
Candida albicans;
demethylzeylasteral;
fluconazole;
synergistic effects;
drug resistance
- From:
Journal of Pharmaceutical Practice and Service
2024;42(4):151-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antifungal effect of demethylzelamaldehyde in vitro. Methods The minimum inhibitory concentrations （MIC） of demethylzeylasteral and fluconazole against 23 fungal strains were determined by micro liquid dilution method. The synergistic index （FICI） of the two drugs was determined using a checkerboard micro liquid dilution method. The synergistic effect of the combination of the two drugs was visually verified by paper diffusion experiments. Finally, the cytotoxicity of demethylzelamaldehyde was determined by CCK-8 method. Results Demethylzelamaldehyde showed a broad spectrum of antifungal activity when used alone, with MICs ranging from 4 g/L to 32 g/L. When combined with fluconazole, the effective concentration of fluconazole could be reduced from over 64 g/L to 0.25 g/L, with FICI values ranging from 0.129 to 0.254, indicating the synergistic effect of the two drugs. The CCK-8 results showed that demethylzeylasteral exhibited cytotoxicity only at concentrations four times higher than the MIC value. Conclusion Demethylzelamaldehyde exhibited good antifungal effect and synergistic effect with fluconazole, and its toxicity was low.
