Upregulation of α1-acidglycoprotein to ameliorate hepatocyte lipid accumulation by Astragaloside derivative HHQ16
10.12206/j.issn.2097-2024.202309026
- VernacularTitle:黄芪甲苷衍生物HHQ16上调α1酸性糖蛋白改善肝细胞脂质沉积
- Author:
Xiang LI
1
;
Xianmin DAI
1
;
Xia LIU
1
;
Yang SUN
1
Author Information
1. Department of Clinical Pharmacy , School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
HHQ16;
ORM;
hepatocyte;
lipid accumulation
- From:
Journal of Pharmaceutical Practice and Service
2024;42(4):141-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of HHQ 16, a derivative of astragaloside Ⅳ, on hepatocyte lipid accumulation. Methods Free fatty acids were used to stimulate lipid hepatocyte accumulation. Triglyceride and Oil Red O staining were detected to reflect hepatocyte lipid accumulation. The expression of α1-acidglycoprotein （ORM） and its regulators were detected by real-time quantitative PCR and immunoblotting. The expression of ORM1 was interfered with siRNA to determine whether it mediated the action of HHQ16.The expression of ORM1 was interfered by siRNA to determine whether it mediated the action of HHQ16. Results HHQ16 significantly ameliorated FFA-induced hepatocyte lipid deposition. HHQ16 elevated ORM expression, and the protective effect of HHQ16 on hepatocyte lipid accumulation was reversed by ORM interference. Conclusion HHQ16 could ameliorate hepatocyte lipid accumulation by elevating ORM.
