Construction and validation of a mouse model with systemic overexpression of human METRNL gene
10.12206/j.issn.2097-2024.202311014
- VernacularTitle:全身过表达人METRNL基因小鼠模型的构建与验证
- Author:
Xuelian WANG
1
;
Sili ZHENG
;
Zhiyong LI
;
Hengyu LUO
;
Chaoyu MIAO
Author Information
1. 上海大学医学院,上海 200444
- Keywords:
METRNL;
systemic overexpression;
mouse
- From:
Journal of Pharmaceutical Practice and Service
2024;42(5):198-202,222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To generate mice with whole-body overexpression of human METRNL gene.Methods Based on Cre-loxP system,Dppa3-Cre mice were mated with Rosa26-LSL-METRNL knock-in mice(R26-LSL-METRNL+/-)to generate R26-L-METRNL+/-mice.The genotypes of the offsprings were identified,and tissues of the blood,heart,liver,spleen,lung,kidney,brain,white adipose and muscle were collected.The expression of human METRNL gene in mice was investigated by quantitative real-time PCR,western blot and enzyme linked immunosorbent assay.Results Compared with wild type control mice,human METRNL in R26-L-METRNL+/-mice significantly expressed at both mRNA and protein levels in tissues,with abundant METRNL protein in blood.Conclusion The mouse model overexpressing human METRNL gene(R26-L-METRNL+/-mouse)was successfully constructed.
