Construction and validation of a mouse model with systemic overexpression of human METRNL gene
10.12206/j.issn.2097-2024.202311014
- VernacularTitle:全身过表达人METRNL基因小鼠模型的构建与验证
- Author:
Xuelian WANG
1
;
Sili ZHENG
2
;
Zhiyong LI
2
;
Hengyu LUO
2
;
Chaoyu MIAO
2
,
3
Author Information
1. School of Medicine, Shanghai University, Shanghai 200444, China.
2. Department of Pharmacology, Naval Medical University, Shanghai 200433, China.
3. School of Medicine, Shanghai University, Shanghai 200444, China
- Keywords:
METRNL;
systemic overexpression;
mouse
- From:
Journal of Pharmaceutical Practice and Service
2024;42(5):198-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To generate mice with whole-body overexpression of human METRNL gene. Methods Based on Cre-loxP system, Dppa3-Cre mice were mated with Rosa26-LSL-METRNL knock-in mice（R26-LSL-METRNL+/-）to generate R26-L-METRNL+/- mice. The genotypes of the offsprings were identified, and tissues of the blood, heart, liver, spleen, lung, kidney, brain, white adipose and muscle were collected. The expression of human METRNL gene in mice was investigated by quantitative real-time PCR, western blot and enzyme linked immunosorbent assay. Results Compared with wild type control mice, human METRNL in R26-L-METRNL+/- mice significantly expressed at both mRNA and protein levels in tissues, with abundant METRNL protein in blood. Conclusion The mouse model overexpressing human METRNL gene（R26-L-METRNL+/- mouse）was successfully constructed.
