Isolation and toxicity of Xanthomonas oryzae pv. oryzae in rice and a collection of  phages against the pathogen

Ngoc Quynh Anh Pham; Vo Thanh Phuc; Pham Do Tra My; Le Thi My Duyen; To Hue Ngoc; Nguyen Minh Thien; Nguyen Hai Duong; Le Thi Thuy Tien; Le Phi Nga; Hoang Anh Hoang

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Isolation and toxicity of Xanthomonas oryzae pv. oryzae in rice and a collection of phages against the pathogen

Author: Ngoc Quynh Anh Pham ^{1
,

2} ; Vo Thanh Phuc ^{1
,

2} ; Pham Do Tra My ^{1
,

2} ; Le Thi My Duyen ^{1
,

2} ; To Hue Ngoc ^{1
,

2} ; Nguyen Minh Thien ^{1
,

2} ; Nguyen Hai Duong ^{1
,

2} ; Le Thi Thuy Tien ^{1
,

2} ; Le Phi Nga ^{1
,

2} ; Hoang Anh Hoang ^{1
,

2}
Author Information

1. Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City, Vietnam.&
2. Vietnam National University Ho Chi Minh City, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
Publication Type:Journal Article
Keywords: Bacterial blight; bacteriophage; biocontrol; stability; toxicity
From:Malaysian Journal of Microbiology 2024;20(no.1):15-23
CountryMalaysia
Language:English
Abstract: Aims:The objective of the study was to isolate bacteriophages and conduct a comprehensive analysis of their potential against Xanthomonas oryzae pv. oryzae (Xoo) strains in the Mekong Delta, Vietnam.
Methodology and results:Twelve Xoo strains were isolated from rice fields located in the Mekong Delta, Vietnam. Among these strains, three strains Xoo L019, L020 and L024, showed the highest disease index of bacterial blight. Four phages specific to Xoo were isolated from soil, water and leaf samples, and their morphologies were determined. In a test against 12 Xoo strains, phage L541, MLA23 or W41 could infect 10 of the 12 Xoo strains, while phage LBH01 could infect 8 of the 12 Xoo strains. The stability of the phages to pH, organic solvents, UV-A and UV-B was also evaluated.
Conclusion, significance and impact of study:The initial characterization of the phages indicates their potential as biocontrol agents against bacterial blight in rice. The study is one of the very first studies about Xoo phages in rice in Vietnam.
Full text:20.2024my0003.pdf