Simultaneous Determination of Artemisinin,Arteannuin B,Chrysosplenetin and Chrysosplenol-D in the Water Extract of Artemisia annua L.by HPLC
10.19378/j.issn.1003-9783.2024.03.015
- VernacularTitle:HPLC法同时测定黄花蒿水提浸膏中青蒿素、青蒿乙素、猫眼草黄素和猫眼草酚D的含量
- Author:
Shijia YUAN
1
;
Shaoqin ZHENG
;
Hujun DU
;
Cuiwen QIU
;
Ruimei LIU
;
Shanyu ZHOU
;
Fei XIAO
;
Yuzheng GU
;
Xiaomeng LU
;
Changsheng DENG
Author Information
1. 广州中医药大学青蒿研究中心,广东 广州 510006
- Keywords:
Artemisia annua L.;
water extract;
artemisinin;
arteannuin B;
chrysosplenetin;
chrysosplenol-D;
content determination;
high performance liquid chromatography
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(3):427-431
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the simultaneous determination of artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L.Methods The analysis was performed on Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column with a mobile phase of acetonitrile(A)-water(B)and the flow rate of 0.8 mL·min-1 in a gradient elution manner.The column temperature was 30℃.The injection volume was 10 μL,and the detection wavelength was 210 nm.Results Artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D were correlated well linearly with peak area in their respective ranges 1.608 8-16.088 μg(r=0.999 9),0.014 1-0.141 4 μg(r=1),0.185 1-1.850 9 μg(r=0.999 9),0.144 1-1.441 4 μg(r=0.999 9),the average recovery rate(n=6)were 102.44%,97.82%,95.07%,95.55%,and the RSD values were 1.12%,1.44%,1.29%,1.53%.Conclusion This method is convenient and accurate.It has good stability and repeatability,and can be used to simultaneously determine the content of artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L.
