Study on HPLC Fingerprint and Multi-Index Components Contents of Atractylodis Macrocephalae Rhizoma
10.19378/j.issn.1003-9783.2024.03.014
- VernacularTitle:白术高效液相色谱指纹图谱及多指标成分含量测定研究
- Author:
Chunqin LI
1
;
Haiyan LING
;
Tuo KAI
;
Andong YANG
;
Jun YANG
Author Information
1. 四川省中医药科学院,四川 成都 610041
- Keywords:
Atractylodis Macrocephalae Rhizoma;
high performance liquid chromatography(HPLC);
multi-wavelength switching;
fingerprint;
cluster analysis;
principal component analysis;
partial least squares-discriminant analysis;
content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(3):419-426
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for simultaneous determination of HPLC fingerprint and multi-target ingredients in Atractylodis Macrocephalae Rhizoma(AMR),in order to provide reference for its quality control.Methods HPLC-DAD multi-wavelength switching method was used to establish fingerprint of AMR,similarity evaluation combined with hierarchical clustering analysis(HCA),principal components analysis(PCA)and discriminant analysis of partial least squares(PLS-DA)were used to carry out chemometric study.The contents of differential component such as atractylenolide Ⅰ,Ⅱ,Ⅲ and atractylon were determined simultaneously.Results The HPLC fingerprint of 37 batches of AMR was established.Nine common peaks were marked,and 4 of them were identified as atractylon,atractylenolide Ⅰ,Ⅱ,Ⅲ.The similarity degrees were between 0.539 and 0.996,the quality of AMR from different origin and different batches varies greatly.Atractylon,atractylenolide Ⅰ,Ⅱ,Ⅲ and one unknown component(peak 9)are the important factors affecting the quality of AMR.Conclusion The combination methods of HPLC fingerprint and simultaneous determinations of multiple components are simple,stable,accurate and reliable,which can provide reference for the quality evaluation of AMR and the improvement of quality standard,as well as lay a foundation for the basic research of its pharmacodynamic substances and related compound.
