The Anti-inflammatory Effects of Qinggan Tongyin Based on the Network Pharmacology and UHPLC-MS/MS
10.19378/j.issn.1003-9783.2024.03.008
- VernacularTitle:基于网络药理学结合UHPLC-MS/MS探讨清感童饮的抗炎作用
- Author:
Haoran HUYAN
1
;
Liwen WANG
;
Xiaoying ZHANG
;
Yue ZHANG
;
Kun ZHOU
Author Information
1. 天津中医药大学中医药研究院,天津 301617
- Keywords:
Qinggan Tongyin;
anti-inflammatory action;
serum pharmacology;
network pharmacology;
UHPLC-MS/MS;
mouse macrophage cells(RAW264.7);
mice
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(3):368-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminary explore the in vitro anti-inflammatory effects of Qinggan Tongyin based on serum pharmacology and network pharmacology.Methods The effects of the serum containing Qinggan Tongyin on the release of NO,cell necrosis factor-α(TNF-α),and interleukin-6(IL-6)in LPS-induced RAW264.7 cells were confirmed using serum pharmacology.UHPLC-MS/MS was used to determine the index components of Qinggan Tongyin.The possible targets and pathways of active components in Qinggan Tongyin for anti-inflammatory properties were predicted by using network pharmacology.Results The results of cellular assay showed that Qinggan Tongyin could dramatically lessen the levels of NO,TNF-α,and IL-6(P＜0.05,P＜0.01,P＜0.001).The higher contents of Qinggan Tongyin were phillyrin A,arctiin,chlorogenic acid,scutellarin,gallic acid,rosmarinic acid,paeoniflorin and phillyrin.A totsl of 215 intersection targets between 17 active components in Qinggan Tongyin and inflammation were obtained,and the 31 core targets were ALB,VEGFA,IL-6,TNF-α,etc..The primary targets can exhibit anti-inflammatory actions by regulating several signaling pathways,such as AGE-RAGE,PI3K-Akt,and MAPK signaling pathway.Conclusion Qinggan Tongyin exerts its anti-inflammatory effects with the characteristic of multiple components and multiple targets.
