Simultaneous Determination of 7 Components in Qingkailing Oral Liquid by HPLC-MS/MS
10.19378/j.issn.1003-9783.2024.02.013
- VernacularTitle:HPLC-MS/MS法同时测定清开灵口服液中7种成分的含量
- Author:
Jinyun WU
1
;
Kaiwei CAI
;
Hongying CHEN
;
Jiaqi WANG
;
Biyan PAN
;
Zhiyong XIE
;
Qiongfeng LIAO
Author Information
1. 广州中医药大学中药学院,广东广州 510006
- Keywords:
Qingkailing Oral Liquid;
adenosine;
chlorogenic acid;
caffeic acid;
geniposide;
baicalin;
hyodeoxycholic acid;
cholic acid;
HPLC-MS/MS;
content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(2):257-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective An HPLC-MS/MS method was established for the simultaneous determination of 7 components in Qingkailing Oral Liquid.Methods The assay was performed on a Waters ACQUITY UPLC BEH C18 column(2.1 mm×10 mm,1.7 μm)and the sample was eluted with a gradient mobile phase containing 10 mmol·L-1 of ammonium acetate and 0.1%of formic acid in water(A)-methanol(B).The mass spectrometry was carried out by electrospray ionization(ESI)with positive/negative ions in multiple reaction monitoring(MRM)mode for quantitative analysis.Results The linear ranges of adenine,chlorogenic acid,caffeic acid,geniposide,baicalin,hyodeoxycholic acid and cholic acid were 0.100 4-3.213,0.784 5-8.982,0.998-3.194,0.622 5-19.92,25.05-300.6,2.513-30.15 and 7.775-93.30 μg·mL-1(r≥0.999 0).The average recoveries(n=6)were 100.9%,98.74%,101.2%,100.2%,100.8%,99.97%and 98.94%with RSD of 1.58%,0.59%,1.78%,1.25%,0.65%,1.69%and 1.07%.The contents of the above mentioned 7 components in 15 tested samples were in the ranges of 0.12-0.18,0.19-0.24,0.06-0.09,0.34-0.37,4.54-4.85,0.49-0.67 and 1.82-2.19 mg·mL-1.The contents of 7 components in tested sample from different manufacturers were closed.Conclusion The method has shown good sensitivity,accuracy,and repeatability.The study can provide reference and data support for the quality control and subsequent research of Qingkailing Oral Liquid.
