Effects of γ-Aminobutyric Acid on the Growth and Related Physiological Metabolism of Plantlets of Tetrastigma hemsleyanum Diels et Gilg From Huaiyu Mountain Under Low Temperature Stress
10.19378/j.issn.1003-9783.2024.01.015
- VernacularTitle:γ-氨基丁酸对怀玉山三叶青低温胁迫下试管苗生长及相关生理代谢的影响
- Author:
Senrong HONG
1
,
2
,
3
,
4
;
Huanyuan YU
;
Ruiling PENG
;
Wenli LIU
;
Tengxin HU
;
Yuting WANG
Author Information
1. 上饶师范学院生命科学学院,江西 上饶 334001
2. 上饶农业技术创新研究院,江西 上饶 334001
3. 上饶市药食同源植物资源保护与利用重点实验室,江西 上饶 334001
4. 上饶市三叶青保育与利用技术创新中心,江西 上饶 334001
- Keywords:
γ-Aminobutyric acid;
Tetrastigma hemsleyanum Diels et Gilg from Huaiyu Mountain;
low temperature stress;
plantlet;
growth;
physiological metabolism
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(1):132-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of γ-aminobutyric acid(GABA)on the growth and related physiological metabolism of plantlets of Tetrastigma hemsleyanum Diels et Gilg from Huaiyu Mountain under low temperature stress,which provides a basis for its application of anti-freezing and cold resistance in the wild-imitation cultivation of T.hemsleyanum Diels et Gilg from Huaiyu mountain.Methods Plantlets of T.hemsleyanum Diels et Gilg from Huaiyu Mountain,which were treated with GABA under low temperature stress,were selected as research objects.The growth indicators,physiological indexes,the relative expression levels of chalcone synthase and flavonol synthase genes were measured using plant tissue culture,physiological and biochemical methods and qRT-PCR,respectively.Results Compared with the control group without GABA,the proliferation coefficient,plant height,fresh weight,dry weight,and tissue water content of the two cultivated plantlets of T.hemsleyanum Diels et Gilg from Huaiyu Mountain("Huaiyu 1"and"Huaiyu 2")reach their maximum value when 0.5 g·L-1 GABA was added to the culture medium.Moreover,content of soluble protein(SP),soluble sugar(SS),proline(Pro),reduced glutathione(GSH),ascorbic acid(AsA),indole acetic acid(IAA),zeatin nucleoside(ZR),gibberellin(GA3),jasmonic acid(JA),the activity of superoxide dismutase(SOD),peroxidase(POD),ascorbic acid peroxidase(APX)and glutathione reductase(GR),and the relative expression level(RQ value)of chalcone synthase(CHS)gene were significantly increased.Moreover,relative conductivity,content of abscisic acid(ABA),hydrogen peroxide(H2O2),malondialdehyde(MDA),rate of O2·-production,and relative expression levels(RQ value)of flavonol synthase(FLS)gene were obviously decreased.The growth of plantlets of T.hemsleyanum Diels et Gilg from Huaiyu Mountain,which were treated with GABA under low temperature stress,was positively correlated with the contents of IAA,ZR,GA3,JA,SS,AsA,GSH,and the expression of CHS genes.Conclusion Appropriate concentration of GABA can regulate the contents of endogenous plant hormones and osmoregulation substances,antioxidant substances,and the expression of key genes in secondary metabolites,so as to promote the growth of plantlets of T.hemsleyanum Diels et Gilg from Huaiyu Mountain under low temperature stress.
