Formulation Optimization of Hydroxyylsafflower Yellow A Nanoparticle Using Box-Behnken Response Surface Method and in Vitro Release Evaluation
10.19378/j.issn.1003-9783.2024.01.014
- VernacularTitle:Box-Behnken响应面法优化羟基红花黄色素A纳米粒处方工艺及体外释放评价
- Author:
Yifei XIAO
1
;
Lixin DU
;
Qidong WEI
;
Huiling LU
;
Zhihua GUO
;
Ya LI
Author Information
1. 湖南中医药大学,湖南 长沙 410208
- Keywords:
Hydroxysafflor yellow A;
PLGA nanoparticles;
Plackett-Burman design experiment;
Box-Behnken response surface method;
in vitro release
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(1):122-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the preparation process of hydroxysafflor yellow A(HSYA)nanoparticle and conduct in vitro release evaluation.Methods HSYA nanoparticles were prepared with PLGA as carrier by modified compound emulsion method.The optimal preparation process of the experiment was selected by Plackett-Burman and Box-Behnken response surface method.The nanoparticles were characterized by using particle size analyzer,TEM scanning electron microscope,Fourier transform infrared spectroscopy(FT-IR),X-ray diffraction(XRD).Frozen(4℃)storage stability,stability in physiological medium,lyophilized protective agent and in vitro release rate were investigated.Results The optimal process prescription of nanoparticle is as follow:pH value is 6.95,the dosage is 2.8 mg,and carrier dosage is 18.2 mg.The size of nanoparticles obtained at optimum condition is(176.4±1.29)nm,the polydiseperse index(PDI)is 0.152±0.014,the Zeta potential is(-17.6±0.46)mV,the encapsulation rate is(78.5±0.49)％,drug loading is(7.3±0.07)％.These nanoparticles showed round and good dispersion.Good stability in 4℃ storage environment and different physiological media of nanoparticles were observed.The best lyophilized protective agent was 1％glucose and the in vitro release rate of nanoparticles at 48 hours was 85％.Conclusion The optimization method is reasonable and reliable.The obtained nanoparticles have good stability and sustained-release effect.The in vitro release behavior conformed to first-order kinetic model.
