Study on Quality Markers of Saorilao Qingfei Zhike Capsules Based on UPLC-QTOF-MS and Network Pharmacology Technology
10.19378/j.issn.1003-9783.2024.01.012
- VernacularTitle:基于UPLC-Q-TOF-MS和网络药理学技术的扫日劳清肺止咳胶囊质量标志物的研究
- Author:
Zhaozuo ZOU
1
;
Tongqiang SHANGGUAN
;
Huajie YANG
;
Yingying LUO
;
Shang LYU
;
Shuangling YANG
;
Xueyan LI
;
Yuying LUO
;
Yi RAO
Author Information
1. 江西中医药大学,江西 南昌 330004
- Keywords:
Saorilao Qingfei Zhike Capsules;
UPLC-QTOF-MS;
network pharmacology technology;
qualitative analysis;
pharmacodynamic experiment;
Q-marker
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(1):103-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)and network pharmacology technology combined with pharmacodynamic experiments were used to analyze the quality markers(Q-markers)in Saorilao Qingfei Zhike Capsules.Methods Using UPLC-Q-TOF-MS technology,the chemical components in different polar extracts of Saorilao Qingfei Zhike Capsules was analyzed.Potential pharmacological components were screened by using antitussive and expectorant models.The"components-targets-diseases"network was constructed and potential Q-markers were screened by network pharmacology technology.Then we conducted pharmacodynamic validation to confirm the Q-markers,which have antitussive and expectorant effects in Saorilao Qingfei Zhike Capsules.Results A total of 120 compounds were obtained from the Saorilao Qingfei Zhike Capsules through qualitative analysis.Among the extracts of different polarity,44 compounds were derived from petroleum ether extract,85 compounds were derived from ethyl acetate extract,79 compounds were derived from n-butanol extract,and 71 compounds were derived from water extract.The results of pharmacological experiments showed that among extracts of different polarity,petroleum ether extract had the best antitussive effect,while n-butanol extract had the best expectorant effect.Three core components for eliminating phlegm and relieving cough were screened through network pharmacology techniques:farcalinol,farcalinediol,and rubimaillin.Pharmacodynamic studies verified that all core components mentioned above have certain antitussive and expectorant effects.Conclusion Based on the above research,farcalinol,farcalindiol,and rubimaillin can be used as Q-markers for the antitussive and expectorant effects of Saorilao Qingfei Zhike Capsules.This paper provides reference for the quality standard of Saorilao Qingfei Zhike Capsules.
