The Osteoprotective Effect of Gusong Yigu Decoction on Ovariectomized Rats Based on PI3K/Akt Signal Pathway
10.19378/j.issn.1003-9783.2023.12.006
- VernacularTitle:基于PI3K/Akt信号通路的骨松益骨方对去势大鼠的骨保护作用
- Author:
Xuxia ZHENG
1
;
Shuang CHAI
;
Liwei WEI
;
Na QIN
Author Information
1. 河南省洛阳正骨医院(河南省骨科医院),河南郑州 450016
- Keywords:
Gusong Yigu Decoction;
postmenopausal osteoporosis(PMOP);
PI3K/Akt signaling pathway;
network pharmacology;
rats
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2023;34(12):1691-1699
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Gusong Yigu Decoction(inchuding Astragali Radix,Codonpsis Radix,Angelicae Sinensis Radix,etc.)in the treatment of postmenopausal osteoporosis(PMOP)based on network pharmacology and animal experiment.Methods The effective ingredients and corresponding targets of Gusong Yigu Decoction were collected by using TCMSP database.GeneCards,TTD,and other databases were used to collect PMOP target proteins.R language was used to obtain the intersection targets and draw Wayne diagram.STRING database was used for the establishment of protein-protein interaction network.At last,GO function enrichment and KEGG pathway enrichment were performed on all common targets.The ovariectomized SD rats were used in the animal experiment.Gusong Yigu Decoction was administered by gavage for 12 weeks.The changes of bone histomorphology were detected by HE staining,the mRNA and protein levels of phosphatidylinositol 3-kinase(PI3K)and protein kinase B(Akt)in bone tissue of proximal tibial were tested by qRT-PCR and Western Blot,respectively.Results A total of 91 effective ingredients of Gusong Yigu Decoction in the treatment of PMOP,70 common targets of drugs-diseases were obtained.GO enrichment analysis mainly included DNA-binding transcription activator activity,RNA polymerase II-specific,ubiquitin protein ligase binding.KEGG pathway enrichment analysis included PI3K/Akt signaling pathway,TNF signaling pathway,and apoptosis.The animal experiment showed that bone histomorphology was significantly improved,meanwhile the mRNA and protein expressions of PI3K and Akt were significantly increased in Gusong Yigu Decoction group(P＜0.01).Conclusion Gusong Yigu Decoction may improve bone microstructure through multiple channels and targets.Gusong Yigu Decoction can increase the number and thickness of bone trabeculae and reduce the separation of bone trabeculae by activating PI3K/Akt signaling pathway,and thus play an anti-osteoporosis role in postmenopausal osteoporosis.
