Sappanone A Plays An Anti-inflammatory Role by Inhibiting the JAK2-STAT3 Signaling Pathway
10.19378/j.issn.1003-9783.2023.12.005
- VernacularTitle:苏木酮A通过抑制JAK2-STAT3信号通路发挥抗炎作用
- Author:
Chengjie DENG
1
;
Hongxing MA
;
Huaxi ZHANG
;
Yuezhou HU
;
Jing HUANG
;
Shiqin SUN
;
Ping XIN
Author Information
1. 哈尔滨医科大学大庆校区药学院,黑龙江大庆 163319
- Keywords:
Sappanone A;
RAW264.7 cell;
anti-inflammation;
JAK2-STAT3;
IL-6
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2023;34(12):1685-1690
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-inflammatory effect and mechanism of sappanone A(SA)on lipopolysaccharide(LPS)-induced RAW264.7 cell model based on JAK2-STAT3 signaling pathway.Methods MTT assay was used to detect the effects of sappanone A,LPS and AG490 on RAW264.7 cell viability.The LPS-induced inflammatory model in RAW264.7 cells was established,and the secretion level of interleukin-6(IL-6)in the supernatant was detected by ELISA.mRNA expressions of IL-6,Janus kinase 2(JAK2)and signal transducer and activator of transcription 3(STAT3)were tested by RT-PCR.The protein expressions of JAK2,phosphorylated JAK2(p-JAK2),STAT3 and phosphorylated STAT3(p-STAT3)were determined by Western Blot.Results Compared with control group,IL-6 secretion level was significantly increased,mRNA expressions of IL-6,JAK2 and STAT3 were up-regulated,and protein expressions of p-JAK2 and p-STAT3 were increased(all P＜0.01)in model group.Compared with model group,high-dosed sappanone A(5 μg·mL-1)was significantly decreased the secretion of IL-6,down-regulated the mRNA expressions of IL-6,JAK2 and STAT3,and inhibited the protein expressions of p-JAK2 and p-STAT3(all P＜0.01).Conclusion Sappanone A may play an anti-inflammatory role by inhibiting the JAK2-STAT3 signaling pathway and hence inhibiting the secretion of IL-6.
