Current status of applying matrigel to in vitro models for cancer reseach
10.3781/j.issn.1000-7431.2023.2308-0457
- VernacularTitle:基质胶应用于肿瘤体外模型的研究现状
- Author:
Yue YANG
1
;
Lijing ZHONG
;
Yifei YANG
;
Gege LI
;
Hongmei CUI
Author Information
1. 兰州大学公共卫生学院毒理学研究所,甘肃兰州 730000
- Keywords:
Matrigel;
Tumor microenvironment;
Tumor modeling;
Decellularization;
Organoid
- From:
Tumor
2023;43(10):813-820
- CountryChina
- Language:Chinese
-
Abstract:
The components of the tumor microenvironment have a complex composition and play an important regulatory role in tumor evolution.The extracellular matrix(ECM)serves as a vital mediator in regulating cancer advancement within the TME.Incorporating ECM into tumor modeling allows for a more comprehensive simulation of the TME in vitro.As a medium for organoid growth,the matrix gel fulfills the functions of ECM.ECM is normally derived from the recombinant basement membrane of mouse sarcoma(Matrigel);however,batch-to-batch variations in Matrigel affect experiment reproducibility.To overcome such issues,researchers have designed synthetic matrix gels.This paper provides an overview of the current research in the application of matrix gels for tumor organoid modeling,offering insights for continuous optimization of organoid culture,thereby achieving more efficient and cost-effective organoid construction and advancing the translation of basic research to clinical applications.
