Mechanism Study of"Atractylodes Macrocephala Koidz-Atractylodes Rhizoma"Drug Pair on the Prevention and Treatment of Hyperlipidemia Based on Network Pharmacology and Transcriptome Analysis
10.16466/j.issn1005-5509.2024.01.002
- VernacularTitle:基于网络药理学及转录组分析的"白术-苍术"药对防治高脂血症作用机制研究
- Author:
Yiting DENGZHANG
1
,
2
;
Jingyi KANG
;
Zhaohuan LOU
Author Information
1. 浙江中医药大学基础医学院 杭州 310053
2. 浙江省中医"瘀毒"证重点实验室
- Keywords:
hyperlipidemia;
Atractylodes macrocephala Koidz;
Atractylodes Rhizoma;
network pharmacology;
drug pair;
PPARγ
- From:
Journal of Zhejiang Chinese Medical University
2024;48(1):9-20
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To clarify the therapeutic effects of the"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair on hyperlipidemia(HLP)and to illuminate the primary mechanism via network pharmacology analysis.[Methods]HLP rat model was established by feeding rats with high-fat diet,and different doses of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair were administrated at the same time.At the end of the experiment,comparing the blood lipid levels of each group of rats and observing the lipid deposition in liver tissue.Using network pharmacology to predict the potential active ingredients and targets of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair for treating HLP,and further experimental verification.[Results]The drug pair of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"had a good effect on anti HLP induced by high-fat diet.Network pharmacology analysis showed that 3[3-acetoatractylodes atractylodes,wogonin,and 3 β-acetoxyatractylodes atractylodes were the potential active ingredients of the drugs in the treatment of HLP,and peroxisome proliferators-activated receptors(PPAR)signaling pathway-related PPARγ and fatty acid binding protein 4(FABP4)were the potential regulatory pathways and targets.The results of the validation experiment further confirmed that the drug pair could significantly up-regulate the expression of PPARy in liver tissue of rats with HLP.[Conclusion]The effect of"Atractylodes macrocephala Koidz-Atractylodes Rhizoma"drug pair on high-fat diet-induced HLP is related to the activation of PPARγ signaling pathway.
