Explore the Mechanism of Huangqi Sijunzi Decoction in the Treatment of Hepatocellular Carcinoma Based on Network Pharma-cology and Bioinformatics
10.16466/j.issn1005-5509.2023.12.004
- VernacularTitle:基于网络药理学和生物信息学探究黄芪四君子汤治疗肝细胞癌的作用机制
- Author:
Junlin CHEN
1
;
Yitian ZHU
;
Yuanjun YIN
Author Information
1. 浙江中医药大学第二临床医学院 杭州 310053
- Keywords:
Huangqi Sijunzi Decoction;
hepatocellular carcinoma;
bioinformatics;
network pharmacology;
cell cycle;
P53 signaling pathway;
apoptosis
- From:
Journal of Zhejiang Chinese Medical University
2023;47(12):1395-1405,1412
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the potential mechanism of action of Huangqi Sijunzi Decoction in the treatment of hepatocellular carcinoma(HCC)by network pharmacology combined with bioinformatics methods.[Methods]The bioactive compounds of drugs and their targets were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database.Differential gene and module gene of HCC were identified by differential analysis and weighted gene co-expression network analysis(WGCNA).On this basis,the network of"Components-targets of Chinese drug"was constructed.Subsequently,the biological function of the target was investigated and protein-protein interaction(PPI)network was constructed to identify key targets for drug therapy of HCC.Finally,the interaction between the target and the compound was explored by molecular docking.[Results]A total of 156 compounds and 227 targets of 5 Chinese drugs were identified,as well as 2 477 and 685 differential genes in The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)datasets,and 2 104 and 2 165 WGCNA association module genes.Then,the Chinese drug component-target network containing 4 drugs,85 compounds and 9 targets was constructed.Biological function analysis showed that the 9 targets were mainly related to cell cycle,p53 signaling pathway and cell senescence.Meanwhile,two core components of kaempferol and quercetin and five core targets of cyclin A2(CCNA2),estrogen receptor alpha(ESR1),cyclin B1(CCNB1),cyclin-dependent kinase 1(CDK1)and topoisomerase Ⅱ alpha(TOP2A)were screened.Molecular docking results showed that the kaempferol and quercetin had stable binding ability to the corresponding core targets.[Conclusion]Huangqi Sijunzi Decoction may play a therapeutic role in HCC by regulating pathways and proteins related with cell cycle.This study provides research ideas and theoretical support for the treatment of HCC with Huangqi Sijunzi Decoction.
