Mechanism of Saffron in Treatment of Parkinson's Disease Based on Network Pharmacology and Molecular Docking
10.16466/j.issn1005-5509.2023.12.003
- VernacularTitle:基于网络药理学和分子对接探讨西红花治疗帕金森病的作用机制
- Author:
Junjie GONG
1
;
Zijin XU
;
Ping WANG
Author Information
1. 义乌市中心医院 浙江,义乌 322099
- Keywords:
Saffron;
Parkinson's disease;
network pharmacology;
molecular docking;
neuroinflammation;
mechanism of action
- From:
Journal of Zhejiang Chinese Medical University
2023;47(12):1383-1394
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the therapeutic effect and molecular mechanism of Chinese medicine Saffron on Parkinson's disease(PD)based on network pharmacology and molecular docking.[Methods]The pharmacodynamic components of Saffron included in the analysis were determined through the pharmacological database and literature research of the Chinese medicine system.With the help of compound target database and disease target database,the corresponding targets of saffron components and PD targets were predicted,respectively.Enrichment analysis of compound-disease intersection targets was made by using R procedures and referencing correlated R-packages.Construction of protein-protein interaction(PPI)networks and analysis of node-related topological parameters.Simulate molecular docking to verify binding properties of representative compounds and key targets.The pharmacodynamic properties of Saffron acid on key targets were preliminarily evaluated by experiments.[Results]The 8 components of saffron with druggable potential corresponded to 279 targets,PD had 2 183 disease targets,and a total of 98 intersection targets of compounds and diseases were obtained.Enrichment analysis showed that the compound-disease intersection target was involved in biological processes such as reactive oxygen radical synthesis and metabolism,neuroinflammation and mitogen-activated protein kinase(MAPK)signaling pathway.The key targets identified by PPI network analysis were tumor necrosis factor(TNF)and signal transducer and activator of transcription 3(STAT3).Molecular docking showed that saffron glycosides and saffron acids had stronger binding activity with TNF.Experiments had shown that saffron acid can reduce the transcription levels of lipopolysaccharide-induced cytoinflammatory factor TNF,interleukin-1β(IL-1β)and inducible nitric oxide oxide synthase(iNOS).[Conclusion]Saffron can be used as a candidate for PD treatment,and its neuroprotective effect may involve the interaction and synergy of many targets and signaling pathways,which deserves special attention to the activity of saffron acid against central nervous system inflammation.
