Activation of monocytes, T-lymphocytes and plasma inflammatory markers in angina patients.
- Author:
Won Ha LEE
1
;
Yoon LEE
;
Jong Ran KIM
;
Jin A CHU
;
Sung Youn LEE
;
Jin Ok JUNG
;
Joon Soo KIM
;
Seonwoo KIM
;
Jung Don SEO
;
Sung S RHEE
;
Jeong Euy PARK
Author Information
1. Clinical Research Center, Samsung Biomedical Research Institute, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
angina;
monocyte;
T-lymphocytes;
inflammation;
C-reactive protein
- MeSH:
Aged;
Angina Pectoris/immunology*;
Angina Pectoris/diagnosis;
Angina, Unstable/immunology*;
Angina, Unstable/diagnosis;
Biological Markers/blood;
C-Reactive Protein/analysis;
Cytokines/blood*;
Female;
HLA-DR Antigens/immunology;
Human;
Interleukins/blood;
Lymphocyte Transformation;
Male;
Middle Age;
Monocyte Chemoattractant Protein-1/blood;
Monocytes/metabolism*;
RNA, Messenger/metabolism;
T-Lymphocytes/metabolism*;
Transforming Growth Factor beta/analysis;
Tumor Necrosis Factor/analysis
- From:Experimental & Molecular Medicine
1999;31(3):159-164
- CountryRepublic of Korea
- Language:English
-
Abstract:
Inflammation and activation of immune cells have important roles in the pathogenesis of atherosclerosis. We analyzed the plasma levels of inflammatory markers and the degree of activation of peripheral blood monocytes and T-lymphocytes isolated from 12 unstable angina, 12 stable angina, and 12 normal subjects. In 20%-33% of patients, monocytes expressed high basal levels of IL-8, tissue factor, IL-1beta, and monocyte chemoattractant protein-1 mRNA. Furthermore, basal mRNA levels of these cytokines showed strong correlation with each other (p < 0.01 in all combination) but not with tumor necrosis factor-alpha or transforming growth factor-beta1. Plasma level of C-reactive protein was highest in the unstable angina patients (1.63+/-0.70 mg/l) and lowest in the control subjects (0.22+/-0.08 mg/l) (P = 0.03). We also observed a high correlation between C-reactive protein level and the occurrence of minor and major coronary events during 6 months of follow-up. Activation status of T-cells, assessed by the percentage of HLA-DR positive cells, was highest in the unstable angina patients (26.8+/-1.4%) compared with that in the control (14.7+/-1.2%) (P = 0.0053). Our data represent the first case showing that the circulating monocytes in angina patients are activated to a state express numerous proatherogenic cytokines. These results may help to diagnose angina patients according to the inflammatory markers and evaluate the prognosis of the disease.
