The application of mass spectrometry flow cytometry and related imaging techniques in the study of the lymphoma microenvironment

VernacularTitle:质谱流式及相关成像技术在淋巴瘤微环境研究中的应用
Author: Ruize CHEN ¹ ; Bohan GU ; Jianyong LI ; Yi XIA
Author Information

1. 南京医科大学第一附属医院，江苏省人民医院血液科，南京医科大学血液研究重点实验室，江苏省肿瘤个体化医学协同创新中心，南京　210029
Keywords: Lymphoma; Microenvironment; Mass spectrometry flow cytometry
From: Chinese Journal of Laboratory Medicine 2024;47(3):341-348
CountryChina
Language:Chinese
Abstract: The tumor microenvironment (TME) is comprised of tumor cells, immune cells and stromal cells, moreover the intricate interactions among these cellular components play a vital role in tumor initiation and progression. Within the TME, immune cells and stromal cells engage in cytotoxic or inflammatory responses to counteract tumor cells, but they employ a range of immune regulatory mechanisms to facilitate tumor evasion. Previous treatments for lymphoma mainly targeted malignant cells themselves. However, with the clinical application of immune checkpoint inhibitors, bispecific antibodies and chimeric antigen receptor T-cell therapy, the elimination of TME-mediated tumor protection has gained increasing attention. By harnessing the high-throughput and multi-dimensional analytical capabilities of mass cytometry (CyTOF) and imaging mass cytometry (IMC), it has become feasible to systematically analyze the composition of the lymphoma TME using large-scale samples.