Single-cell RNA sequencing-based research progress analysis of microglia in diabetic retinopathy
10.3760/cma.j.cn511434-20230703-00287
- VernacularTitle:基于单细胞转录组测序技术探讨小胶质细胞在糖尿病视网膜病变中的研究进展
- Author:
Fangcheng XU
1
;
Haixiang WU
Author Information
1. 复旦大学附属眼耳鼻喉科医院眼科，上海 200031
- Keywords:
Microglia;
Diabetic retinopathy;
Heterogeneity;
Single-cell RNA sequencing;
Review
- From:
Chinese Journal of Ocular Fundus Diseases
2024;40(2):165-169
- CountryChina
- Language:Chinese
-
Abstract:
Diabetic retinopathy (DR) is one of the main causes of vision loss and irreversible blindness in the working-age population, closely regarded as the destruction of the retinal neurovascular unit (NVU). As an important component of the NVU, retinal microglia (RMG) plays a vital role in the progression of DR. In recent years, single-cell RNA sequencing (scRNA-seq) technology has emerged as an important tool in transcriptomic analysis. This latest method reveals the heterogeneity and complexity of RNA transcriptional profiles within individual cells, as well as the composition of different cell types and functions. Utilizing scRNA-seq technology, researchers have further revealed the role of RMG in the occurrence and development of DR, discovering phenotypic heterogeneity, regional heterogeneity, and cell-to-cell communication in RMG. It is anticipated that in the future, more omics technologies and multi-omics correlation analysis methods will be applied to DR and even other ophthalmic diseases, exploring potential diagnostic and therapeutic targets, providing different perspectives for the clinical diagnosis, treatment, and scientific research of DR, and truly promoting clinical translation through technological innovation, thereby benefiting patients with DR diseases.
