Comparative analysis of anti-Helicobacter pylori activities of FEMY-R7 composed of Laminaria japonica and Oenothera biennis extracts in mice and humans.
- Author:
Tae Su KIM
1
;
Kyungha SHIN
;
Joseph H JEON
;
Ehn Kyoung CHOI
;
Youngjin CHOI
;
Sung Pyo LEE
;
Yoon Bok LEE
;
Yun Bae KIM
Author Information
- Publication Type:Original Article
- Keywords: Helicobacter pylori; FEMY-R7; Laminaria japonica; Oenothera biennis; Campylobcter-like organism-detection test; bacterial identification
- MeSH: Animals; Anti-Bacterial Agents; Bacteria; Capsules; Feces; Functional Food; Gastric Mucosa; Helicobacter; Helicobacter pylori; Humans; Laminaria*; Male; Mice*; Oenothera biennis*
- From:Laboratory Animal Research 2015;31(1):7-12
- CountryRepublic of Korea
- Language:English
- Abstract: Helicobacter pylori-eliminating effects of FEMY-R7, composed of Laminaria japonica and Oenothera biennis extracts, were investigated in mice and humans. Male C57BL/6 mice were infected with the bacteria by intragastric inoculation (1x10(9) CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with total 20, 64 or 200 mg/kg/day FEMY-R7 for 2 weeks. In Campylobcter-like organism (CLO)-detection tests on gastric mucosa and feces, FEMY-R7 reduced the urease-positive reactivity in a dose-dependent manner; i.e., the positivity ratios were decreased to 70, 20, and 10% for gastric mocosa and to 80, 50, and 20% for feces. In a clinical sudy, human subjects, confirmed to be infected with Helicobacter pylori, were orally administered twice a day with capsules containing total 100, 320 or 1,000 mg/man/day FEMY-R7 (matching doses for 20, 64 or 200 mg/kg/day, respectively, in mice from a body surface area-based dose translation) for 8 weeks. FEMY-R7 decreased the positivity ratios in feces to 70, 40, and 30%, respectively. In bacterial culture, H. pylori was identified from the CLO-positive stools of mice and humans. The bacterial identification ratios exhibited a good correlation between the matching doses in mice and humans. It is suggested that FEMY-R7 could be a promising functional food without tolerance as an adjunct to reduce the dosage of antibiotics for the treatment of recurrent H. pylori infection.
