Effects of β-glucan on biological functions of mouse bone marrow-dendritic cells induced under different conditions
10.3760/cma.j.cn112309-20230609-00165
- VernacularTitle:β-葡聚糖对不同诱导来源的小鼠骨髓树突状细胞生物学功能的影响
- Author:
Xuehan DUAN
1
;
Yongzhe HAO
;
Jun DING
;
Chunjian QI
Author Information
1. 南京医科大学附属常州市第二人民医院中心实验室，常州 213164
- Keywords:
β-glucan;
Dendritic cells;
Immune function
- From:
Chinese Journal of Microbiology and Immunology
2024;44(3):225-233
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of β-glucan on the activation and functions of mouse bone marrow-derived dendritic cells (BMDCs) induced under different conditions.Methods:Mouse bone marrow from the tibia and fibula was in vitro induced into FL-DCs and GM-DCs by FMS-like tyrosine kinase 3 ligand (Flt-3L) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in combination with IL-4 into, respectively. These cells were stimulated with whole glucan particles (WGP) and flow cytometry was performed to detect the expression of surface molecules such as CD40, CD80, MHCⅠ, MHCⅡ, CD8α, and CD11b on them. ELISA was used to detect the levels of IL-12p40, TNF-α, IL-6, and IL-10 in cell culture supernatants. The expression of each cytokine and chemokine receptor at the mRNA level was detected by real-time fluorescence quantitative PCR. CD8 + and CD4 + T cells in OT-Ⅰ and OT-Ⅱ mice were sorted out by magnetic bead sorting kit and co-cultured with FL-DCs and GM-DCs in the presence of ovalbumin (OVA). And then, the proliferation and differentiation of T cells were detected by flow cytometry. Results:WGP significantly promoted the expression of CD40, CD80, MHCⅠ, MHCⅡ and CD8α on FL-DCs and GM-DCs ( P<0.05). The secretion of IL-12p40 and TNF-α by FL-DCs and GM-DCs was significantly enhanced after WGP treatment ( P<0.05), while there are significant differences in the secretion of IL-6 and IL-10 between FL-DCs and GM-DCs. Real-time fluorescence quantitative PCR showed that WGP promoted the expression of chemokine receptors CXCR5 and CCR7 by GM-DCs ( P<0.001 and P<0.01), and the expression of CCR7 by FL-DCs ( P<0.0001). WGP promoted CD4 + T cells to secrete more IFN-γ and IL-17α when they were co-cultured with FL-DCs or GM-DCs ( P<0.05). Conclusions:WGP induces the maturation of BMDCs and improves the ability of BMDCs to activate effector T cells. Besides, WGP prompts the differentiation of BMDCs that are induced under different conditions to different Th cells.
