Transcriptomic analysis of A549 cells infected with ON1 genotype of human respiratory syncytial virus subtype A isolated in Beijing
10.3760/cma.j.cn112309-20240112-00022
- VernacularTitle:人呼吸道合胞病毒北京地区A亚型ON1基因型地方株感染A549细胞转录组学分析
- Author:
Fengjie WANG
1
;
Mingli JIANG
;
Qi GUO
;
Xiaoyun LI
;
Yu SUN
;
Runan ZHU
;
Linqing ZHAO
Author Information
1. 首都儿科研究所病毒学研究室，儿童病毒病病原学北京市重点实验室，北京 100020
- Keywords:
Human respiratory syncytial virus;
Local strain;
ON1 genotype;
Transcriptomic analysis;
Differentially expressed genes
- From:
Chinese Journal of Microbiology and Immunology
2024;44(2):128-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the differentially expressed genes of human respiratory syncytial virus (RSV) subtype A genotype ON1, a predominant genotype in Beijing, after infecting A549 cells using transcriptomic sequencing, and provide potential targets for RSV prevention and treatment.Methods:A local strain (61397-ON1) identified by whole-genome sequencing as ON1 genotype of RSV subtype A was selected to infect A549 cells. Total mRNA was extracted, and the differentially expressed genes in infected and uninfected A549 cells were screened by transcriptomic sequencing. GO analysis and KEGG pathway analysis were performed. Besides, six genes with differential expression ratio greater than two times were randomly selected for qRT-PCR verification.Results:There were 1 632 differentially expressed genes between infected and uninfected A549 cells, of which 807 genes were up-regulated and 825 genes were down-regulated. The differentially expressed genes were mainly involved in immune response-related biological processes such as cytokine response and positive regulation of MAPK cascades, and were enriched in MAPK signaling pathway, NOD-like receptor signaling pathway, p53 signaling pathway, TNF signaling pathway, IL-17 signaling pathway and NF-κB signaling pathway. The results of qRT-PCR for six differentially expressed genes were consistent with the trend of transcriptome data.Conclusions:The differentially expressed genes of RSV A subtype ON1 genotype after infecting A549 cells are mainly involved in cytokine response and immune-related signaling pathways. This study provides basic data for further study of the molecular mechanism of RSV infection and the development of prevention and treatment strategies.
