Genetic variation and evolution of influenza viruses isolated from co-infection cases in Guangdong Province
10.3760/cma.j.cn112309-20230717-00213
- VernacularTitle:广东省流感病毒亚型双重感染病例分离株的基因变异和进化分析
- Author:
Lijun LIANG
1
;
Jing TAN
;
Yushi HUANG
;
Huishi DENG
;
Jianxiang YU
;
Lirong ZOU
;
Huan ZHANG
;
Qianfang GUO
;
Zhencui LI
;
Bosheng LI
Author Information
1. 广东省疾病预防控制中心病原微生物检验所，广州 511430
- Keywords:
Co-infection;
Influenza virus;
Hemagglutinin;
Neuraminidase
- From:
Chinese Journal of Microbiology and Immunology
2023;43(11):829-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze and reveal the genetic evolution and variation of influenza viruses in cases of co-infection in Guangdong Province.Methods:Throat swab samples were collected from four cases of H1N1pdm and H3N2 co-infection for viral isolation. The isolated strains were subjected to antigen analysis and neuraminidase inhibitor susceptibility test. High-throughput sequencing was used to detect the sequences of strains in three throat swab samples and one virus strain, and then genetic variations were analyzed.Results:Four influenza viruses were isolated with one strain of H1N1pdm and three of H3N2 subtype, and all of them were genetically similar to the vaccine strain in 2022-2023. The HA genes of H1N1pdm and H3N2 strains belonged to clade 6B.1A.5a.2a and 2a.3a.1, respectively. The isolated strains belonged to the same clade as the strains prevalent in Guangdong during the same period. No drug-resistant variations were detected in N1 or N2 gene, and the isolated strains were sensitive to oseltamivir and zanamivir.Conclusions:H1pdm subtype had stronger replication ability than H3 subtype in the influenza viruses isolated from co-infected cases. H1N1pdm and H3N2 subtype influenza viruses were genetically similar to the strains circulating in Guangdong at the same time. The isolated H1N1pdm and H3N2 strains were sensitive to both oseltamivir and zanamivir, indicating that they could continue to be used in the treatment of influenza virus infections caused by one or two genotypes.
