The role of iron metabolism in aerobic exercise′s inhibition of cardiomyocyte apoptosis in atherosclerosis
10.3760/cma.j.issn.0254-1424.2024.01.002
- VernacularTitle:铁代谢在有氧运动改善动脉粥样硬化小鼠心肌细胞凋亡中的作用机制研究
- Author:
Xihua LIU
1
;
Tiantian MA
;
Fang QIN
;
Zifu YU
;
Jie GAO
Author Information
1. 山东中医药大学附属医院，济南 250014
- Keywords:
Aerobic exercise;
Atherosclerosis;
Myocardium;
Iron metabolism;
Apoptosis
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2024;46(1):7-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To demonstrate any role of iron metabolism in the inhibition by aerobic exercise of myocardial apoptosis in atherosclerotic mice.Methods:Eight-week-old male ApoE -/- gene knockout mice were randomly divided into a control group, a model group and an aerobic exercise group, each of 9. A model of atherosclerosis was induced in the rats of the model and aerobic exercise groups by feeding them a " western" diet for 12 weeks. During that time the aerobic exercise group only was given aerobic exercise training. The control group was fed normal rat chow during that period. Myocardial apoptosis was detected using TUNEL staining, and the expression and localization of ferritin heavy chain 1 (FTH1) and glutathione peroxidase 4 (GPX4) in the myocardium used immunohistochemistry. Western blotting was applied to detect the FTH1 and GPX4 protein levels, and iron deposition in the myocardium was detected using Prussian blue staining. Iron, lipid peroxide malondialdehyde (MDA) and glutathione peroxidase (GSH-PX) in the myocardial tissue were also measured. Results:The TUNEL staining showed significant apoptosis in the model group. In the aerobic exercise group it was significantly less. There was obvious iron deposition in the myocardia of the model group, which was significantly reduced in the aerobic exercise group. The average FTH1 and GPX4 levels in the model group were lower than in the control group, and significantly elevated in the aerobic exercise group.in the aerobic exercise group than in the model group. Iron and MDA levels in the aerobic exercise group were significantly lower, on average, than among the model group, while that of GSH-PX was significantly higher.Conclusions:Aerobic exercise can significantly inhibit cardiomyocyte apoptosis in atherosclerotic mice. The mechanism may be closely related to better iron metabolism, reduced oxidative stress and the inhibition of iron overload.
