Acteoside improves renal fibrosis in rats with unilateral ureteral obstruction
10.3760/cma.j.cn441217-20221226-01230
- VernacularTitle:毛蕊花糖苷改善单侧输尿管梗阻模型大鼠肾纤维化
- Author:
Xin LIN
1
;
Ying XIE
;
Licheng JIANG
;
Rong DONG
;
Jiali YU
;
Jing YUAN
;
Yan ZHA
Author Information
1. 贵州省人民医院肾内科，贵阳 550002
- Keywords:
Acteoside;
Fibrosis;
Ureteral obstruction;
Myofibroblast;
Extracellular matrix;
Apoptosis
- From:
Chinese Journal of Nephrology
2023;39(10):778-782
- CountryChina
- Language:Chinese
-
Abstract:
The study explored the effect of acteoside on renal fibrosis in rats with unilateral ureteral obstruction. Twenty-four healthy adult male SD rats were randomly divided into sham (CON) group, unilateral ureteral obstruction (UUO) group, UUO+acteoside (ACT) group, UUO+benazepril (BZ) group by random number table method. The animal model of renal fibrosis was established by unilateral ureteral ligation. The pathological changes of renal tissues in each group of rats were observed by HE staining and Masson staining. Immunohistochemical method was used to detect the protein expression levels of collagenⅢ, heat shock protein (HSP)-47, connective tissue growth factor (CTGF), α-smooth muscle actin (α-SMA), β-catenin, Bcl-2, and caspase-3. Western blotting was used to detect the protein expression of glucose-regulated protein 78 (GRP78) in renal tissue. Compared with CON group, UUO group had renal tubular dilation and significantly increased interstitial collagen fiber deposition; The degree of renal tubular dilation and interstitial collagen fibers in UUO+ACT group and UUO+BZ group were significantly reduced compared to UUO group, but still significantly heavier than CON group. Compared with CON group, the protein expression levels of collagenⅢ, HSP-47, CTGF, α-SMA, β-catenin and GRP78 in renal tissues of UUO group were significantly higher, while the protein expression level of Bcl-2 was significantly lower (all P<0.05). In comparison with UUO group, the expression levels of Bcl-2 in UUO+ACT group and UUO+BZ group were significantly higher, while the expression levels of other proteins were all significantly lower (all P<0.05). The study shows that acteoside may improve renal fibrosis by decreasing the expression levels of collagenⅢ, HSP-47, CTGF, α-SMA, β-catenin and GRP78, and upregulating the expression level of Bcl-2 in renal tissues.
