Effect of Preconditioning by Low-Flow Perfusion on Cardioprotection.
10.4070/kcj.2001.31.12.1252
- Author:
Ho Dirk KIM
1
Author Information
1. Department of Anatomy, Sungkyunkwan University School of Medicine, Suwon, Korea. hdkim@med.skku.ac.kr
- Publication Type:Original Article
- Keywords:
Ischemic preconditioning;
Myocardial infarction;
Phosphates;
Lactic acid
- MeSH:
Adenosine Triphosphate;
Energy Metabolism;
Heart;
Hydrolysis;
Ischemia;
Ischemic Preconditioning;
Lactic Acid;
Myocardial Infarction;
Perfusion*;
Phosphates;
Reperfusion
- From:Korean Circulation Journal
2001;31(12):1252-1260
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Recent reports suggest that brief periods of low-flow ischemia (lf Isc) and reperfusion (R) before prolonged Isc mimic ischemic preconditioning (IPC) in murine hearts, probably by a preservation of high energy phosphates. MATERIALS AND METHODS: In order to test this hypothesis, Langendorff-perfused isolated rabbit hearts were subjected to 45 min lf Isc (5% of baseline perfusion flow) with lf IPC or not, followed by 120 min R. lf IPC was induced by a single episode of 5 min lf Isc and 10 min R. These were compared with IPC hearts by 5 min no-flow Isc and 10 min R. RESULTS: lf IPC as well as IPC enhanced post-ischemic functional recovery although IPC did not reduce infarct size (Isc control, 37.5+/-3.1, IPC, 16.2+/-1.5, lf Isc, 43.0+/-0.7, and lf IPC, 40.4+/-1.0% of the left ventricle). Myocardial ATP hydrolysis and lactate production during the preischemic, ischemic, and reperfusion periods were not differ between the experimental groups. CONCLUSION: These results suggest that a brief period of lf Isc could not precondition the rabbit heart and the energy metabolism hypothesis may not be a universal mechanism for the cardioprotective effect of IPC.