Lamin B2 promotes migration of retroperitoneal liposarcoma cells by regulating AKT signaling pathway
10.3760/cma.j.cn113855-20230116-00025
- VernacularTitle:核纤层蛋白B2通过调控AKT信号通路促进腹膜后脂肪肉瘤细胞的迁移
- Author:
Shixiang MA
1
;
Mengmeng XIAO
;
Xiangji LI
;
Chenghua LUO
Author Information
1. 北京大学国际医院腹膜后肿瘤外科，北京 102206
- Keywords:
Liposarcoma;
Cell migration assays;
Gene expression regulation
- From:
Chinese Journal of General Surgery
2024;39(1):51-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Lamins B2 (LMNB2) on the migration of human retroperitoneal liposarcoma (RPLS) cells SW872.Methods:Immunohistochemistry was used to analyze the the differential expression levels of LMNB2 in 33 RPLS tissue samples . The correlation between LMNB2 expression and clinical prognosis and clinicopathological features was analyzed. siRNA was used to lower the expression level of LMNB2 in tumor cells, and the effect of LMNB2 on the scratch healing ability and migration ability of SW872 cells was examined by using wound-healing assay and transwell migration assay. The expression levels of p-AKT and AKT in each group cells were detected by Western blot.Results:Patients with high LMNB2 expression had a lower recurrence-free survival and overall survival compared to those with low LMNB2 expression, and were more likely to experience recurrence, ( χ2=4.872, P=0.027; χ2=4.180, P=0.041; χ2=7.127, P=0.008). The migration ability of cells was significantly reduced following the silencing of LMNB2 expression ( t=11.240, P<0.01; t=7.445, P<0.01). The expression level of p-AKT in the silencing group was significantly lower than that in the control group, while there was no significant difference in the expression level of AKT between the two groups ( t=9.784, P<0.01). Conclusion:LMNB2 may promote the migration of human retroperitoneal liposarcoma cells SW872 by regulating AKT signaling pathway.
