Effects of Zhuangyao Shuanglu Tongnao Formula on neuronal apoptosis of rats with ischemia-reperfusion induced injury
10.3969/j.issn.1001-1528.2024.03.013
- VernacularTitle:壮药双路通脑方对脑缺血再灌注损伤大鼠神经元凋亡的影响
- Author:
Yang ZHAI
1
,
2
;
Xue-Ni MO
;
Hong-Li TENG
;
Yue-Qiang HU
;
Guang-Shan ZHENG
;
Wei MA
;
Peng YANG
;
Xiao-Ping MEI
;
Min ZOU
;
Kai-Hua WANG
Author Information
1. 广西国际壮医医院脑病科,广西 南宁 530200
2. 广西中医药大学,广西 南宁 530200
- Keywords:
Zhuangyao Shuanglu Tongnao Formula;
ischemic stroke;
cerebral ischemia-reperfusion injury;
apoptosis;
oxidative stress;
inflammatory response;
TLR4/NF-κB signaling pathway;
Nrf2/HO-1 signaling pathway
- From:
Chinese Traditional Patent Medicine
2024;46(3):795-802
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of Zhuangyao Shuanglu Tongnao Formula on neuronal apoptosis in rats with cerebral ischemia-reperfusion injury based on the study of oxidative stress and inflammatory response.METHODS The rats were randomly divided into the sham operation group,the model group,the edaravone group(3.0 mg/kg),the low,medium and high dose groups(9.0,18.0,36.0 g/kg)of Zhuangyao Shuanglu Tongnao Formula,with 18 rats in each group.The middle cerebral artery occlusion/reperfusion was conducted by thread embolism method to simulate cerebral ischemia reperfusion injury in rats followed by 6 days corresponding drugs administration.Subsequently,the rats had their neurological function deficit scored by Zeal Longa scoring method;their sizes of cerebral infarction areas measured by TTC staining;their pathological damage and apoptosis of neurons in hippocampal CA1 area of ischemic penumbra of the brain tissue detected by HE staining and TUNEL staining;their SOD activity and levels of GSH,MDA,IL-6,IL-1β,TNF-α in brain tissue detected by kits;and their protein expressions of Bax,Bcl-2,caspase-3,cleaved-capase-3,TLR4,NF-κB p65,Nrf2,HO-1 in rat brain tissue determined by Western blot.RESULTS Compared with the model group,the groups intervened with edaravone,medium and high dose of Zhuangyao Shuanglu Tongnao Formula displayed improvements in the scores of nerve function defects,the rate of cerebral infarction,the rate of neuronal apoptosis,the levels of IL-6,IL-1β,TNF-α and MDA in the ischemic penumbra of brain tissues,the protein expressions of Bax and TLR4,the ratio of cleaved-capase-3/caspase-3 and p-NF-κB p65/NF-κB p65(P＜0.05),the levels of GSH,the activity of SOD and the protein expressions of Bcl-2,Nrf2 and HO-1(P＜0.05).CONCLUSION Being an inhibitor of oxidative stress and inflammatory response,Zhuangyao Shuanglu Tongnao Formula can alleviate brain injury in rats with cerebral ischemia reperfusion injury through the inhibition of neuronal apoptosis and improvement of neural function mediated by the inhibition of TLR4/NF-κB signal pathway and activation of Nrf2/HO-1 signal pathway.
