Variation rules of main secondary metabolites in Hedysari Radix before and after rubbing strip
10.3969/j.issn.1001-1528.2024.03.007
- VernacularTitle:红芪搓条前后主要次级代谢产物变化规律研究
- Author:
Xu-Dong LUO
1
;
Xin-Rong LI
;
Cheng-Yi LI
;
Peng QI
;
Ting-Ting LIANG
;
Shu-Bin LIU
;
Zheng-Ze QIANG
;
Jun-Gang HE
;
Xu LI
;
Xiao-Cheng WEI
;
Xiao-Li FENG
;
Ming-Wei WANG
Author Information
1. 甘肃中医药大学,甘肃 兰州 730000
- Keywords:
Hedysari Radix;
rubbing strip;
secondary metabolites;
UPLC-MS/MS;
cluster analysis;
principal component analysis;
orthogonal partial least squares discriminant analysis
- From:
Chinese Traditional Patent Medicine
2024;46(3):747-754
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the variation rules of main secondary metabolites in Hedysari Radix before and after rubbing strip.METHODS UPLC-MS/MS was adopted in the content determination of formononetin,ononin,calycosin,calycosin-7-glucoside,medicarpin,genistein,luteolin,liquiritigenin,isoliquiritigenin,vanillic acid,ferulic acid,γ-aminobutyric acid,adenosine and betaine,after which cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used for chemical pattern recognition to explore differential components.RESULTS After rubbing strip,formononetin,calycosin,liquiritigenin and γ-aminobutynic acid demonstrated increased contents,along with decreased contents of ononin,calycosin-7-glucoside and vanillic acid.The samples with and without rubbing strip were clustered into two types,calycosin-7-glucoside,formononetin,γ-aminobutynic acid,vanillic acid,calycosin-7-glucoside and formononetin were differential components.CONCLUSION This experiment clarifies the differences of chemical constituents in Hedysari Radix before and after rubbing strip,which can provide a reference for the research on rubbing strip mechanism of other medicinal materials.
