Quality standard improvement for Guanxin Shengmai Pills
10.3969/j.issn.1001-1528.2024.03.003
- VernacularTitle:冠心生脉丸质量标准提升
- Author:
Jing-Yu LIU
1
;
Moo-Seob KIM
;
Li-Hua GU
;
Li-Hong WU
;
Zheng-Tao WANG
Author Information
1. 上海中医药大学中药研究所,中药标准化教育部重点实验室,国家中医药管理局中药新资源与质量评价重点实验室,上海 201203
- Keywords:
Guanxin Shengmai Pills;
Ginseng Radix et Rhizoma;
Notoginseng Radix et Rhizoma;
ginsenosides;
qualitative identification;
content determination;
TLC;
HPLC
- From:
Chinese Traditional Patent Medicine
2024;46(3):724-729
- CountryChina
- Language:Chinese
-
Abstract:
AIM To improve the quality standard for Guanxin Shengmai Pills.METHODS TLC was adopted in the qualitative identification of Ginseng Radix et Rhizoma and Notoginseng Radix et Rhizoma,the analysis was performed on a silica G thin layer plate,along with the low layer solution of chloroform-methanol-water(13 : 7 : 2)stood at below 10℃ as a mobile phase,and 10％sulfuric acid ethanol solution as a derivatization reagent.HPLC was applied to determining the contents of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd,the analysis was performed on a 20℃ thermostatic Thermo Accucore-C18 column(4.6 mm×150 mm,2.6 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 203 nm.RESULTS The clear TLC bands present without negative interference.Four constituents showed good linear relationships within their own ranges(R2≥0.999 9),whose average recoveries were 91.21％-106.86％with the RSDs of 0.68％-1.43％.CONCLUSION This specific and reproducible method can provide a reference for the quality control of Guanxin Shengmai Pills.
