Identification of chemical components of Longmu Qingxin Mixture by UPLC-Q-TOF-MS and research on its material basis for attention deficit hyperactivity disorder
10.3969/j.issn.1001-1528.2024.02.023
- VernacularTitle:基于UPLC-Q-TOF-MS的龙牡清心合剂成分鉴定及其治疗注意缺陷多动障碍的物质基础研究
- Author:
Xue-Jun LI
1
;
Zhi-Yan JIANG
;
Zhen XIAO
;
Xiu-Feng CHEN
;
Shu-Min WANG
;
Yi-Xing ZHANG
;
Wen-Yan PU
Author Information
1. 上海中医药大学附属龙华医院儿科, 上海 200032
- Keywords:
Longmu Qingxin Mixture;
attention deficit hyperactivity disorder;
UPLC-Q-TOF-MS;
fingerprint;
chemical composition;
pharmacological action
- From:
Chinese Traditional Patent Medicine
2024;46(2):490-498
- CountryChina
- Language:Chinese
-
Abstract:
AIM To identify the chemical components of Longmu Qingxin Mixture by UPLC-Q-TOF-MS and study its material basis for the treatment of attention deficit hyperactivity disorder.METHODS The sample was detected by mass spectrometry in positive and negative ion mode on a Waters CORTECS? UPLC? T3 chromatographic column.The data were analyzed with Peakview 1.2 software and matched with the Natural Products HR-MS/MS Spectral Library 1.0 database,and the components were identified in combination with literature reports.The material basis of Longmu Qingxin Mixture for the treatment of attention deficit hyperactivity disorder was analysed according to the identified components.RESULTS Forty chemical components were identified,including 11 flavonoids,6 monoterpene glycosides,4 triterpene saponins,3 phenolic acids,6 alkaloids etc.,which mainly derived from Radix Astragali,Radix Paeoniae Alba,Radix Scutellariae,licorice root,Ramulus Uncariae cum,etc.,baicalein,formononetin,astragaloside Ⅳ and rhynchophylline may be the material basis for the therapeutic effect of Longmu Qingxin Mixture.CONCLUSION UPLC-Q-TOF-MS can quickly identify the chemical components of Longmu Qingxin Mixture.Flavonoids,triterpene saponins and alkaloids may be the material basis for Longmu Qingxin Mixture for the treatment of attention deficit hyperactivity disorder,which can provide the basis for its material basis research,quality standard establishment and pharmacological study of the dismantled formula.
