Secondary metabolites and their α-glucosidase inhibitory activity of endophyte fungi from Orixa japonica
10.3969/j.issn.1001-1528.2024.02.021
- VernacularTitle:臭常山内生真菌次级代谢产物及其α-糖苷酶抑制活性研究
- Author:
Pei-Feng ZHOU
1
;
Yong-Zhong LU
;
Xiao-Jie WANG
;
Yan LI
;
Zhen ZHANG
Author Information
1. 贵州大学药学院, 贵州 贵阳 550025
- Keywords:
Orixa japonica Thunb.;
endophyte fungi;
chemical constituents;
isolation and identification;
α-glucosidase inhibitory activities
- From:
Chinese Traditional Patent Medicine
2024;46(2):478-483
- CountryChina
- Language:Chinese
-
Abstract:
AIM To identify the endophytic fungus G-(JK)-2 from Orixa japonica Thunb.and to study its secondary metabolites and their α-glucosidase inhibitory activities.METHODS Through the ITS sequence,the evolutionary tree that identifies the endophytic fungus G-(JK)-2 was established.Then 45 days rice solid medium of endophytic fungus G-(JK)-2 was extracted by methanol,and then by ethyl acetate.The ethyl acetate extract was separated and purified by silica gel chromatography,Sephadex LH-20,and semi-preparative HPLC.The structures of obtained compounds were identified by physicochemical properties and spectral data.Their α-glucosidase inhibitory activities were evaluated by PNPG method.RESULTS The endophytic fungus G-(JK)-2 from O.japonica was identified as Fusarium nematophilum.Thirteen compounds were isolated and identified as p-hydroxybenzaldehyde(G1),4-hydroxyacetophenone(G2),anhydromevalonolactone(G3),flazine(G4),salicylic acid(G5),p-hydroxybenzoic acid(G6),di-(2-ethylhexyl)-phthalate(G7),terephthalic acid bis(2-ethyl-hexyl)ester(G8),thymine(G9),uridine(G10),adenosine(G11),2′-deoxyuridine(G12),nicotinic acid(G13).The inhibitory effect of each compound on α-glucosidase was in sequence of G4>G11>G10>G13>G12.CONCLUSION All compounds are first isolated from the endophytic fungi of the O.japonica,and G10,G11,G13 are first isolated from the endophytic fungi of Fusarium.G4 and G11 have mild inhibition to α-glucosidase.
