Establishment of HPLC characteristic chromatogram and quantitative transmission laws for Baqi Rougan Decoction reference sample
10.3969/j.issn.1001-1528.2024.02.004
- VernacularTitle:巴芪柔肝方基准样品HPLC特征图谱建立及其量值传递规律研究
- Author:
Sai-Long GENG
1
;
Qin ZHOU
;
Shui-Gen SUN
;
Man LI
;
Li-Jie ZHAO
;
Ji-Quan ZHANG
;
Yi FENG
Author Information
1. 上海中医药大学创新中药研究院, 中药现代制剂技术教育部工程研究中心, 上海 201203
- Keywords:
Baqi Rougan Decoction;
reference sample;
HPLC characteristic chromatogram;
quantitative transmission laws
- From:
Chinese Traditional Patent Medicine
2024;46(2):370-378
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC characteristic chromatogram of Baqi Rougan Decoction reference sample,and to investigate its quantitative transmission laws.METHODS The contents of calycosin 7-O-glucoside,hesperidin,rosmarinic acid,curcumenol and nystose were determined.The transfer rates of decoction piece-aqueous decoction-reference sample were calculated,after which the paste-forming rate and pH value were recorded.RESULTS There were sixteen characteristic peaks in fifteen batches of reference samples with the similarities of 0.90,nine of which were identified.The average transfer rates of nystose and calycosin 7-O-glucoside in the reference sample were(83.14±6.25)%and(77.81±8.31)%,while those of rosmarinic acid and curcumenol in the aqueous decoction-reference sample were(81.71±6.27)%and(72.16±5.91)%,along with the average paste-forming rate and pH value of(38.91%±1.46%)and 5.13±0.08,respectively.CONCLUSION This stable and feasible method can provide a reference for the selection of preparation process and evaluation of key chemical properties for Baqi Rougan Decoction.
