Simultaneous content determination of seventeen constituents in Yangxue Ruanjian Capsules by UPLC-MS/MS
10.3969/j.issn.1001-1528.2024.02.001
- VernacularTitle:UPLC-MS/MS法同时测定养血软坚胶囊中17种成分的含量
- Author:
Yong-Ming LIU
1
,
2
;
Shu-Sen LIU
;
Yi-Zhe XIONG
;
Xiang WANG
;
Yu-Yun WU
;
Jin LIU
;
Ling-Yun PAN
;
Guo-Qing DU
;
Hong-Sheng ZHAN
Author Information
1. 上海中医药大学附属曙光医院石氏伤科医学中心, 上海 200021
2. 上海市中医药研究院骨伤科研究所, 上海 201203
- Keywords:
Yangxue Ruanjian Capsules;
chemical constituents;
content determination;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(2):353-358
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of liquiritin apioside,alibiflorin,swertiamarin,methyl gallate,benzoylpaeoniflorin,sweroside,6′-O-β-D-glucosylgentiopicroside,isoliquiritigenin,loganic acid,liquiritigenin,gallic acid,paeoniflorin,oxypaeoniflorin,gentiopicroside,glycyrrhizic acid,isoliquiritoside and liquiritin in Yangxue Ruanjian Capsules.METHODS The analysis was performed on a 40℃thermostatic Waters BEH C18column(2.1 mm×100 mm,1.7 μm),with the mobile phase comprising of 2 mmol/L ammonium acetate(containing 0.1%formic acid)-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Seventeen constituents showed good linear relationships within their own ranges(r>0.999 6),whose average recoveries were 91.33%-104.03%with the RSDs of 1.58%-3.50%.CONCLUSION This rapid,accurate and stable method can be used for the quality control of Yangxue Ruanjian Capsules.
