Neoflavonoids from Dalbergia cochinchinensis and their anti-hypoxia/reoxygenation injury activities on H9c2 myocardial cells
10.3969/j.issn.1001-1528.2024.01.022
- VernacularTitle:交趾黄檀新黄酮类成分及其抗H9c2心肌细胞缺氧/复氧损伤活性研究
- Author:
Jia-Hui REN
1
;
Qi-Wan ZHENG
;
Xiao-Wei MENG
;
Yan PANG
;
Lan-Ying CHEN
;
Rong-Hua LIU
Author Information
1. 江西中医药大学药学院, 江西 南昌 330004
- Keywords:
Dalbergia cochinchinensis Pierre ex Laness;
neoflavonoids;
isolation and identification;
H9c2 myocardial cells;
hypoxia/reoxygenation injury;
structure-activity relationship
- From:
Chinese Traditional Patent Medicine
2024;46(1):126-132
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the neoflavonoids from Dalbergia cochinchinensis Pierre ex Laness and their anti-hypoxia/reoxygenation injury activities on H9c2 myocardial cells.METHODS The 70%ethanol extract from D.cochinchinensis was isolated and purified by silica gel,Sephadex LH-20 and reverse-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The CCK-8 method was used to detect their activities on H9c2 cells and protective effects on hypoxia-reoxygenation injury of H9c2 cells,and their structure-activity relationship was analyzed.RESULTS Twelve compounds were isolated and identified as latifolin(1),5-O-methyllatifolin(2),mimosifoliol(3),5-O-methydalbergiphenol(4),dalbergiphenol(5),cearoin(6),2,4-dihydroxy-5-methoxy-benzophenone(7),2-hydroxy-4,5-dimethoxybenzophenone(8),melannoin(9),2,2′,5-trihydroxy-4-methoxybenzophenone(10),dalbergin(11),4-methoxydalbergione(12).The dalbergiphenols and dalbergins had little toxicity to H9c2 cells,and dalbergiphenols had strong activity against hypoxia-reoxygenation injury of H9c2 cells.CONCLUSION Compound 8 is a new natural product.Compounds 4,9 are isolated from this plant for the first time.Dalbergiphenols may be the main neoflavonoids against hypoxia-reoxygenation injury of H9c2 cells.
